Please use this identifier to cite or link to this item: https://doi.org/10.1089/ars.2019.7850
Title: Cysteine Deprivation Targets Ovarian Clear Cell Carcinoma Via Oxidative Stress and Iron-Sulfur Cluster Biogenesis Deficit
Authors: WISNA NOVERA 
LEE ZHENG WEI 
NIN SIJIN,DAWN 
Dai, MZY
SHABANA BINTE IDRES 
Wu, H
Damen, JMA
Tan Tuan Zea 
SIM YI LOONG, ARTHUR 
LONG YUN CHAU 
Wu, W
HUANG YUN JU,RUBY 
DENG LIH WEN 
Keywords: cystathionase
cysteine
iron−sulfur cluster
mitochondria
oxidative stress
system xc−
Issue Date: 10-Dec-2020
Publisher: Mary Ann Liebert Inc
Citation: WISNA NOVERA, LEE ZHENG WEI, NIN SIJIN,DAWN, Dai, MZY, SHABANA BINTE IDRES, Wu, H, Damen, JMA, Tan Tuan Zea, SIM YI LOONG, ARTHUR, LONG YUN CHAU, Wu, W, HUANG YUN JU,RUBY, DENG LIH WEN (2020-12-10). Cysteine Deprivation Targets Ovarian Clear Cell Carcinoma Via Oxidative Stress and Iron-Sulfur Cluster Biogenesis Deficit. Antioxidants and Redox Signaling 33 (17) : 1191-1208. ScholarBank@NUS Repository. https://doi.org/10.1089/ars.2019.7850
Abstract: Aims: Current treatment options for ovarian clear cell carcinoma (OCCC) are limited to combination of platinum-based and other cytotoxic agents to which patients respond poorly due to intrinsic chemoresistance. There is therefore an urgent need to develop alternative therapeutic strategies for OCCC. Results: Cysteine deprivation suppresses OCCC growth in vitro and in vivo with no apparent toxicity. Modes of cell death induced by cysteine deprivation in OCCC are determined by their innate metabolic profiles. Cysteine-deprived glycolytic OCCC is abolished primarily by oxidative stress-dependent necrosis and ferroptosis, which can otherwise be prevented by pretreatment with antioxidative agents. Meanwhile, OCCC that relies on mitochondria respiration for its bioenergetics is suppressed through apoptosis, which can otherwise be averted by pretreatment with cysteine precursor alone, but not with antioxidative agents. Cysteine deprivation induces apoptosis in respiring OCCC by limiting iron-sulfur (Fe-S) cluster synthesis in the mitochondria, without which electron transport chain may be disrupted. Respiring OCCC responds to Fe-S cluster deficit by increasing iron influx into the mitochondria, which leads to iron overload, mitochondria damage, and eventual cell death. Innovation/Conclusion: This study demonstrates the importance of cysteine availability in OCCC that is for its antioxidative property and its less appreciated role in mitochondria respiration. Regardless of OCCC metabolic profiles, cysteine deprivation abolishes both glycolytic and respiring OCCC growth in vitro and in vivo. Conclusion: This study highlights the therapeutic potential of cysteine deprivation for OCCC.
Source Title: Antioxidants and Redox Signaling
URI: https://scholarbank.nus.edu.sg/handle/10635/187460
ISSN: 1523-0864
1557-7716
DOI: 10.1089/ars.2019.7850
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