Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pntd.0008910
Title: Adenovirus vectored IFN-alpha protects mice from lethal challenge of Chikungunya virus infection
Authors: Chen, Huixin 
Min, Nyo 
Ma, Luyao 
Mok, Chee-Keng 
Chu, Justin Jang Hann 
Keywords: Science & Technology
Life Sciences & Biomedicine
Infectious Diseases
Parasitology
Tropical Medicine
EQUINE ENCEPHALITIS-VIRUS
NERVOUS-SYSTEM
INTERFERON
REPLICATION
DISEASE
Issue Date: 2020
Publisher: PUBLIC LIBRARY SCIENCE
Citation: Chen, Huixin, Min, Nyo, Ma, Luyao, Mok, Chee-Keng, Chu, Justin Jang Hann (2020/12/01). Adenovirus vectored IFN-alpha protects mice from lethal challenge of Chikungunya virus infection. PLOS NEGLECTED TROPICAL DISEASES 14 (12). ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pntd.0008910
Abstract: Chikungunya virus (CHIKV) is a mosquito-borne pathogen that is responsible for numerous large and geographical epidemics, causing millions of cases. However, there is no vaccine or therapeutics against CHIKV infection available. Interferon-alpha (IFN-α) has been shown to produce potent antiviral responses during viral infection. Herein we demonstrated the use of an adenovirus-vectored expressed mouse IFN-α (mDEF201) as a prophylactic and therapeutic treatment against CHIKV in vivo. 6-day-old BALB/c mice were pre- or post-treated intranasally with single dose of mDEF201 at 5 x 106 PFU per mouse and challenged with lethal dose of CHIKV. Complete survival protection was observed in mice upon a single dose of mDEF201 administration 1 days prior to virus challenge. Viral load in the serum and multiple organs were significantly reduced upon mDEF201 administration in a dose dependent manner as compare with adenovirus 5 vector placebo set. Histological analysis of the mice tissue revealed that mDEF201 could significantly reduce the tissue morphological abnormities, mainly infiltration of immune cells and muscle fibre necrosis caused by CHIKV infection. In addition, administration of mDEF201 at 6 hours post CHIKV challenge also showed promising inhibitory effect against viral replication and dissemination. In conclusion, single-dose of intranasal administration with mDEF201 as a prophylactic or therapeutic agent within 6 hours post CHIKV infection is highly protective against a lethal challenge of CHIKV in the murine model.
Source Title: PLOS NEGLECTED TROPICAL DISEASES
URI: https://scholarbank.nus.edu.sg/handle/10635/185669
ISSN: 19352735
19352735
DOI: 10.1371/journal.pntd.0008910
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