Please use this identifier to cite or link to this item: https://doi.org/10.1080/19490976.2020.1801964
Title: A compromised developmental trajectory of the infant gut microbiome and metabolome in atopic eczema
Authors: Ta L.D.H.
Chan J.C.Y.
Yap G.C. 
Purbojati R.W. 
Drautz-Moses D.I.
Koh Y.M.
Tay C.J.X.
Huang C.-H.
Kioh D.Y.Q.
Woon J.Y.
Tham E.H. 
Loo E.X.L.
Shek L.P.C. 
Karnani N.
Goh A.
Van Bever H.P.S. 
Teoh O.H.
Chan Y.H.
Lay C. 
Knol J.
Yap F.
Tan K.H.
Chong Y.-S. 
Godfrey K.M.
Kjelleberg S.
Schuster S.C.
Chan E.C.Y.
Lee B.W.
Keywords: allergen sensitization
atopic dermatitis
atopic eczema
Early life
gut metabolome
gut microbiome
SCFA
Issue Date: 7-Oct-2020
Publisher: Taylor & Francis
Citation: Ta L.D.H., Chan J.C.Y., Yap G.C., Purbojati R.W., Drautz-Moses D.I., Koh Y.M., Tay C.J.X., Huang C.-H., Kioh D.Y.Q., Woon J.Y., Tham E.H., Loo E.X.L., Shek L.P.C., Karnani N., Goh A., Van Bever H.P.S., Teoh O.H., Chan Y.H., Lay C., Knol J., Yap F., Tan K.H., Chong Y.-S., Godfrey K.M., Kjelleberg S., Schuster S.C., Chan E.C.Y., Lee B.W. (2020-10-07). A compromised developmental trajectory of the infant gut microbiome and metabolome in atopic eczema. Gut Microbes 12 (1) : 1-22. ScholarBank@NUS Repository. https://doi.org/10.1080/19490976.2020.1801964
Abstract: Evidence is accumulating that the establishment of the gut microbiome in early life influences the development of atopic eczema. In this longitudinal study, we used integrated multi-omics analyses to infer functional mechanisms by which the microbiome modulates atopic eczema risk. We measured the functionality of the gut microbiome and metabolome of 63 infants between ages 3 weeks and 12 months with well-defined eczema cases and controls in a sub-cohort from the Growing Up in Singapore Toward healthy Outcomes (GUSTO) mother-offspring cohort. At 3 weeks, the microbiome and metabolome of allergen-sensitized atopic eczema infants were characterized by an enrichment of Escherichia coli and Klebsiella pneumoniae, associated with increased stool D-glucose concentration and increased gene expression of associated virulence factors. A delayed colonization by beneficial Bacteroides fragilis and subsequent delayed accumulation of butyrate and propionate producers after 3 months was also observed. Here, we describe an aberrant developmental trajectory of the gut microbiome and stool metabolome in allergen sensitized atopic eczema infants. The infographic describes an impaired developmental trajectory of the gut microbiome and metabolome in allergen-sensitized atopic eczema (AE) infants and infer its contribution in modulating allergy risk in the Singaporean mother-offspring GUSTO cohort. The key microbial signature of AE is characterized by (1) an enrichment of Escherichia coli and Klebsiella pneumoniae which are associated with accumulation of pre-glycolysis intermediates (D-glucose) via the trehalose metabolic pathway, increased gene expression of associated virulence factors (invasin, adhesin, flagellin and lipopolysaccharides) by utilizing ATP from oxidative phosphorylation and delayed production of butyrate and propionate, (2) depletion of Bacteroides fragilis which resulted in lower expression of immunostimulatory bacterial cell envelope structure and folate (vitamin B9) biosynthesis pathway, and (3) accompanied depletion of bacterial groups with the ability to derive butyrate and propionate through direct or indirect pathways which collectively resulted in reduced glycolysis, butyrate and propionate biosynthesis. © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.
Source Title: Gut Microbes
URI: https://scholarbank.nus.edu.sg/handle/10635/184721
ISSN: 19490976
DOI: 10.1080/19490976.2020.1801964
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Figure 4 - Proposed Pathway.tif26.39 MBTIFF

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Figure 5 - Intermediate Metabolites.tif22.11 MBTIFF

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Figure 6 - Early Species Bacteria_Compilation.tif52.43 MBTIFF

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Supplementary Tables - Ta et al - Gut Microbes 010620.pdf608.26 kBAdobe PDF

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Figure 1 - Major SCFAs.tif21.71 MBTIFF

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Figure 2 - Family Bacteria_Compilation.tif48.76 MBTIFF

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Figure 3 - Metagenomics_Metabolomics - Landscape.tif44.75 MBTIFF

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