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Title: Determination of oxidized phosphatidylcholines by hydrophilic interaction liquid chromatography coupled to fourier transform mass spectrometry
Authors: Sala, P
Pötz, S
Brunner, M
Trötzmüller, M
Fauland, A
Triebl, A 
Hartler, J
Lankmayr, E
Köfeler, H.C
Keywords: low density lipoprotein
oxidized low density lipoprotein
collisionally activated dissociation
drug determination
Fourier transform mass spectrometry
hydrophilic interaction chromatography
ion trap mass spectrometry
sensitivity and specificity
tandem mass spectrometry
validation process
chemical phenomena
electrospray mass spectrometry
Fourier analysis
high performance liquid chromatography
isolation and purification
oxidation reduction reaction
Chromatography, High Pressure Liquid
Fourier Analysis
Hydrophobic and Hydrophilic Interactions
Lipoproteins, LDL
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Issue Date: 2015
Citation: Sala, P, Pötz, S, Brunner, M, Trötzmüller, M, Fauland, A, Triebl, A, Hartler, J, Lankmayr, E, Köfeler, H.C (2015). Determination of oxidized phosphatidylcholines by hydrophilic interaction liquid chromatography coupled to fourier transform mass spectrometry. International Journal of Molecular Sciences 16 (4) : 8351-8363. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: A novel liquid chromatography-mass spectrometry (LC-MS) approach for analysis of oxidized phosphatidylcholines by an Orbitrap Fourier Transform mass spectrometer in positive electrospray ionization (ESI) coupled to hydrophilic interaction liquid chromatography (HILIC) was developed. This method depends on three selectivity criteria for separation and identification: retention time, exact mass at a resolution of 100,000 and collision induced dissociation (CID) fragment spectra in a linear ion trap. The process of chromatography development showed the best separation properties with a silica-based Kinetex column. This type of chromatography was able to separate all major lipid classes expected in mammalian samples, yielding increased sensitivity of oxidized phosphatidylcholines over reversed phase chromatography. Identification of molecular species was achieved by exact mass on intact molecular ions and CID tandem mass spectra containing characteristic fragments. Due to a lack of commercially available standards, method development was performed with copper induced oxidation products of palmitoyl-arachidonoyl-phosphatidylcholine, which resulted in a plethora of lipid species oxidized at the arachidonoyl moiety. Validation of the method was done with copper oxidized human low-density lipoprotein (LDL) prepared by ultracentrifugation. In these LDL samples we could identify 46 oxidized molecular phosphatidylcholine species out of 99 possible candidates. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
Source Title: International Journal of Molecular Sciences
ISSN: 16616596
DOI: 10.3390/ijms16048351
Rights: Attribution 4.0 International
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