Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep27085
Title: Drosophila cells use nanotube-like structures to transfer dsRNA and RNAi machinery between cells
Authors: Karlikow, M
Goic, B
Mongelli, V
Salles, A
Schmitt, C
Bonne, I 
Zurzolo, C
Saleh, M.-C
Keywords: actin
AGO2 protein, Drosophila
argonaute protein
double stranded RNA
Drosophila protein
enhanced green fluorescent protein
fluorescent protein 583
green fluorescent protein
photoprotein
Rab protein
Rab7 protein
tubulin
viral protein
animal
antagonists and inhibitors
cell communication
cell line
cell organelle
Dicistroviridae
Drosophila melanogaster
gene expression regulation
genetics
growth, development and aging
metabolism
microbiology
Pectobacterium carotovorum
reporter gene
RNA interference
transport at the cellular level
ultrastructure
virology
Actins
Animals
Argonaute Proteins
Biological Transport
Cell Communication
Cell Line
Dicistroviridae
Drosophila melanogaster
Drosophila Proteins
Gene Expression Regulation
Genes, Reporter
Green Fluorescent Proteins
Luminescent Proteins
Organelles
Pectobacterium carotovorum
rab GTP-Binding Proteins
RNA Interference
RNA, Double-Stranded
Tubulin
Viral Proteins
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Karlikow, M, Goic, B, Mongelli, V, Salles, A, Schmitt, C, Bonne, I, Zurzolo, C, Saleh, M.-C (2016). Drosophila cells use nanotube-like structures to transfer dsRNA and RNAi machinery between cells. Scientific Reports 6 : 27085. ScholarBank@NUS Repository. https://doi.org/10.1038/srep27085
Rights: Attribution 4.0 International
Abstract: Tunnelling nanotubes and cytonemes function as highways for the transport of organelles, cytosolic and membrane-bound molecules, and pathogens between cells. During viral infection in the model organism Drosophila melanogaster, a systemic RNAi antiviral response is established presumably through the transport of a silencing signal from one cell to another via an unknown mechanism. Because of their role in cell-cell communication, we investigated whether nanotube-like structures could be a mediator of the silencing signal. Here, we describe for the first time in the context of a viral infection the presence of nanotube-like structures in different Drosophila cell types. These tubules, made of actin and tubulin, were associated with components of the RNAi machinery, including Argonaute 2, double-stranded RNA, and CG4572. Moreover, they were more abundant during viral, but not bacterial, infection. Super resolution structured illumination microscopy showed that Argonaute 2 and tubulin reside inside the tubules. We propose that nanotube-like structures are one of the mechanisms by which Argonaute 2, as part of the antiviral RNAi machinery, is transported between infected and non-infected cells to trigger systemic antiviral immunity in Drosophila.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/182465
ISSN: 2045-2322
DOI: 10.1038/srep27085
Rights: Attribution 4.0 International
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