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https://doi.org/10.1038/srep27085
Title: | Drosophila cells use nanotube-like structures to transfer dsRNA and RNAi machinery between cells |
Authors: | Karlikow, M Goic, B Mongelli, V Salles, A Schmitt, C Bonne, I Zurzolo, C Saleh, M.-C |
Keywords: | actin AGO2 protein, Drosophila argonaute protein double stranded RNA Drosophila protein enhanced green fluorescent protein fluorescent protein 583 green fluorescent protein photoprotein Rab protein Rab7 protein tubulin viral protein animal antagonists and inhibitors cell communication cell line cell organelle Dicistroviridae Drosophila melanogaster gene expression regulation genetics growth, development and aging metabolism microbiology Pectobacterium carotovorum reporter gene RNA interference transport at the cellular level ultrastructure virology Actins Animals Argonaute Proteins Biological Transport Cell Communication Cell Line Dicistroviridae Drosophila melanogaster Drosophila Proteins Gene Expression Regulation Genes, Reporter Green Fluorescent Proteins Luminescent Proteins Organelles Pectobacterium carotovorum rab GTP-Binding Proteins RNA Interference RNA, Double-Stranded Tubulin Viral Proteins |
Issue Date: | 2016 |
Publisher: | Nature Publishing Group |
Citation: | Karlikow, M, Goic, B, Mongelli, V, Salles, A, Schmitt, C, Bonne, I, Zurzolo, C, Saleh, M.-C (2016). Drosophila cells use nanotube-like structures to transfer dsRNA and RNAi machinery between cells. Scientific Reports 6 : 27085. ScholarBank@NUS Repository. https://doi.org/10.1038/srep27085 |
Rights: | Attribution 4.0 International |
Abstract: | Tunnelling nanotubes and cytonemes function as highways for the transport of organelles, cytosolic and membrane-bound molecules, and pathogens between cells. During viral infection in the model organism Drosophila melanogaster, a systemic RNAi antiviral response is established presumably through the transport of a silencing signal from one cell to another via an unknown mechanism. Because of their role in cell-cell communication, we investigated whether nanotube-like structures could be a mediator of the silencing signal. Here, we describe for the first time in the context of a viral infection the presence of nanotube-like structures in different Drosophila cell types. These tubules, made of actin and tubulin, were associated with components of the RNAi machinery, including Argonaute 2, double-stranded RNA, and CG4572. Moreover, they were more abundant during viral, but not bacterial, infection. Super resolution structured illumination microscopy showed that Argonaute 2 and tubulin reside inside the tubules. We propose that nanotube-like structures are one of the mechanisms by which Argonaute 2, as part of the antiviral RNAi machinery, is transported between infected and non-infected cells to trigger systemic antiviral immunity in Drosophila. |
Source Title: | Scientific Reports |
URI: | https://scholarbank.nus.edu.sg/handle/10635/182465 |
ISSN: | 2045-2322 |
DOI: | 10.1038/srep27085 |
Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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