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Title: | Weibel-Palade body size modulates the adhesive activity of its von Willebrand Factor cargo in cultured endothelial cells | Authors: | Ferraro, F Da Silva, M.L Grimes, W Lee, H.K Ketteler, R Kriston-Vizi, J Cutler, D.F |
Keywords: | von Willebrand factor cell adhesion cell culture cell membrane cell organelle endothelium cell exocytosis genetics human metabolism pathology secretory vesicle thrombocyte Weibel Palade body Blood Platelets Cell Adhesion Cell Membrane Cells, Cultured Endothelial Cells Exocytosis Humans Organelles Secretory Vesicles von Willebrand Factor Weibel-Palade Bodies |
Issue Date: | 2016 | Publisher: | Nature Publishing Group | Citation: | Ferraro, F, Da Silva, M.L, Grimes, W, Lee, H.K, Ketteler, R, Kriston-Vizi, J, Cutler, D.F (2016). Weibel-Palade body size modulates the adhesive activity of its von Willebrand Factor cargo in cultured endothelial cells. Scientific Reports 6 : 32473. ScholarBank@NUS Repository. https://doi.org/10.1038/srep32473 | Rights: | Attribution 4.0 International | Abstract: | Changes in the size of cellular organelles are often linked to modifications in their function. Endothelial cells store von Willebrand Factor (vWF), a glycoprotein essential to haemostasis in Weibel-Palade bodies (WPBs), cigar-shaped secretory granules that are generated in a wide range of sizes. We recently showed that forcing changes in the size of WPBs modifies the activity of this cargo. We now find that endothelial cells treated with statins produce shorter WPBs and that the vWF they release at exocytosis displays a reduced capability to recruit platelets to the endothelial cell surface. Investigating other functional consequences of size changes of WPBs, we also report that the endothelial surface-associated vWF formed at exocytosis recruits soluble plasma vWF and that this process is reduced by treatments that shorten WPBs, statins included. These results indicate that the post-exocytic adhesive activity of vWF towards platelets and plasma vWF at the endothelial surface reflects the size of their storage organelle. Our findings therefore show that changes in WPB size, by influencing the adhesive activity of its vWF cargo, may represent a novel mode of regulation of platelet aggregation at the vascular wall. © 2016 The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/182432 | ISSN: | 2045-2322 | DOI: | 10.1038/srep32473 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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