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Title: The global regulator Crc plays a multifaceted role in modulation of type III secretion system in Pseudomonas aeruginosa
Authors: Dong, Y.-H
Zhang, X.-F
Zhang, L.-H 
Keywords: Crc protein
RNA binding protein
unclassified drug
bacterial gene
controlled study
down regulation
gene expression
gene expression profiling
gene expression regulation
gene mutation
genetic transcription
priority journal
protein function
Pseudomonas aeruginosa
real time polymerase chain reaction
regulatory mechanism
signal transduction
T3SS gene
type III secretion system
Bacterial Proteins
Bacterial Secretion Systems
Gene Expression Regulation, Bacterial
Gene Knockout Techniques
Pseudomonas aeruginosa
Real-Time Polymerase Chain Reaction
Repressor Proteins
Signal Transduction
Issue Date: 2013
Citation: Dong, Y.-H, Zhang, X.-F, Zhang, L.-H (2013). The global regulator Crc plays a multifaceted role in modulation of type III secretion system in Pseudomonas aeruginosa. MicrobiologyOpen 2 (1) : 161-172. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The opportunistic pathogen Pseudomonas aeruginosa utilizes type III secretion system (T3SS) to translocate effector proteins into eukaryotic host cells that subvert normal host cell functions to the benefit of the pathogen, and results in serious infections. T3SS in P. aeruginosa is controlled by a complex system of regulatory mechanisms and signaling pathways. In this study, we described that Crc, an RNA-binding protein, exerts a positive impact on T3SS in P. aeruginosa, as evidenced by promoter activity assays of several key T3SS genes, transcriptomics, RT-PCR, and immunoblotting in crc mutant. We further demonstrated that the regulatory function of Crc on the T3SS was mediated through the T3SS master regulator ExsA and linked to the Cbr/Crc signaling system. Expression profiling of the crc mutant revealed a downregulation of flagship T3SS genes as well as 16 other genes known to regulate T3SS gene expression in P. aeruginosa. On the basis of these data, we proposed that Crc may exert multifaceted control on the T3SS through various pathways, which may serve to fine-tune this virulence mechanism in response to environmental changes and nutrient sources. © 2012 The Authors.
Source Title: MicrobiologyOpen
ISSN: 20458827
DOI: 10.1002/mbo3.54
Rights: Attribution 4.0 International
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