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https://doi.org/10.1186/2051-5960-2-61
Title: | Intracellular A? pathology and early cognitive impairments in a transgenic rat overexpressing human amyloid precursor protein: A multidimensional study | Authors: | Iulita, M.F Allard, S Richter, L Munter, L.M Ducatenzeiler, A Weise, C DoCarmo, S Klein, W.L Multhaup, G Cuello, A.C |
Keywords: | amyloid beta protein amyloid beta protein[1-40] amyloid beta-protein (1-42) amyloid precursor protein peptide fragment secretase animal auditory stimulation brain cerebrospinal fluid cognitive defect conditioning disease model fear gene expression regulation genetics human intracellular fluid metabolism mutation pain measurement pathology physiology rat recognition regression analysis transgenic rat adverse effects Acoustic Stimulation Amyloid beta-Peptides Amyloid beta-Protein Precursor Amyloid Precursor Protein Secretases Animals Brain Cognition Disorders Conditioning (Psychology) Disease Models, Animal Fear Gene Expression Regulation Humans Intracellular Fluid Mutation Pain Measurement Peptide Fragments Rats Rats, Transgenic Recognition (Psychology) Regression Analysis |
Issue Date: | 2014 | Citation: | Iulita, M.F, Allard, S, Richter, L, Munter, L.M, Ducatenzeiler, A, Weise, C, DoCarmo, S, Klein, W.L, Multhaup, G, Cuello, A.C (2014). Intracellular A? pathology and early cognitive impairments in a transgenic rat overexpressing human amyloid precursor protein: A multidimensional study. Acta Neuropathologica Communications 2 (1) : 61. ScholarBank@NUS Repository. https://doi.org/10.1186/2051-5960-2-61 | Rights: | Attribution 4.0 International | Abstract: | Numerous studies have implicated the abnormal accumulation of intraneuronal amyloid-? (A?) as an important contributor to Alzheimer's disease (AD) pathology, capable of triggering neuroinflammation, tau hyperphosphorylation and cognitive deficits. However, the occurrence and pathological relevance of intracellular A? remain a matter of controversial debate. In this study, we have used a multidimensional approach including high-magnification and super-resolution microscopy, cerebro-spinal fluid (CSF) mass spectrometry analysis and ELISA to investigate the A? pathology and its associated cognitive impairments, in a novel transgenic rat model overexpressing human APP. Our microscopy studies with quantitative co-localization analysis revealed the presence of intraneuronal A? in transgenic rats, with an immunological signal that was clearly distinguished from that of the amyloid precursor protein (APP) and its C-terminal fragments (CTFs). The early intraneuronal pathology was accompanied by a significant elevation of soluble A?42 peptides that paralleled the presence and progression of early cognitive deficits, several months prior to amyloid plaque deposition. A?38, A?39, A?40 and A?42 peptides were detected in the rat CSF by MALDI-MS analysis even at the plaque-free stages; suggesting that a combination of intracellular and soluble extracellular A? may be responsible for impairing cognition at early time points. Taken together, our results demonstrate that the intraneuronal development of AD-like amyloid pathology includes a mixture of molecular species (A?, APP and CTFs) of which a considerable component is A?; and that the early presence of these species within neurons has deleterious effects in the CNS, even before the development of full-blown AD-like pathology. © 2014 Iulita et al.; licensee BioMed Central Ltd. | Source Title: | Acta Neuropathologica Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/182028 | ISSN: | 20515960 | DOI: | 10.1186/2051-5960-2-61 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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