Please use this identifier to cite or link to this item: https://doi.org/10.1186/1472-6882-11-65
Title: High catechin concentrations detected in Withania somnifera (ashwagandha) by high performance liquid chromatography analysis
Authors: Alam, N
Hossain, M
Khalil, M.D
Moniruzzaman, M 
Sulaiman, S.A
Gan, S.H
Keywords: 1,1 diphenyl 2 picrylhydrazyl
benzoic acid
catechin
flavonoid
gallic acid
kaempferol
naringenin
para coumaric acid
phenol derivative
scavenger
syringic acid
vanillic acid
Withania somnifera extract
plant extract
antioxidant activity
article
chemical composition
concentration (parameters)
drug determination
drug isolation
drug potency
fruit
high performance liquid chromatography
nonhuman
plant leaf
plant root
spectrophotometry
Withania somnifera
chemistry
high performance liquid chromatography
isolation and purification
Withania
Catechin
Chromatography, High Pressure Liquid
Phenols
Plant Extracts
Withania
Issue Date: 2011
Citation: Alam, N, Hossain, M, Khalil, M.D, Moniruzzaman, M, Sulaiman, S.A, Gan, S.H (2011). High catechin concentrations detected in Withania somnifera (ashwagandha) by high performance liquid chromatography analysis. BMC Complementary and Alternative Medicine 11 : 65. ScholarBank@NUS Repository. https://doi.org/10.1186/1472-6882-11-65
Rights: Attribution 4.0 International
Abstract: Background: Withania somnifera is an important medicinal plant traditionally used in the treatment of many diseases. The present study was carried out to characterize the phenolic acids, flavonoids and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activities in methanolic extracts of W. somnifera fruits, roots and leaves (WSFEt, WSREt and WSLEt).Methods: WSFEt, WSREt and WSLEt was prepared by using 80% aqueous methanol and total polyphenols, flavonoids as well as DPPH radical scavenging activities were determined by spectrophotometric methods and phenolic acid profiles were determined by HPLC methods.Results: High concentrations of both phenolics and flavonoids were detected in all parts of the plant with the former ranging between 17.80 ± 5.80 and 32.58 ± 3.16 mg/g (dry weight) and the latter ranging between 15.49 ± 1.02 and 31.58 ± 5.07 mg/g. All of the three different plant parts showed strong DPPH radical scavenging activities (59.16 ± 1.20 to 91.84 ± 0.38%). Eight polyphenols (gallic, syringic, benzoic, p-coumaric and vanillic acids as well as catechin, kaempferol and naringenin) have been identified by HPLC in parts of the plant as well. Among all the polyphenols, catechin was detected in the highest concentration (13.01 ± 8.93 to 30.61 ± 11.41 mg/g).Conclusion: The results indicating that W. somnifera is a plant with strong therapeutic properties thus further supporting its traditional claims. All major parts of W. somnifera such as the roots, fruits and leaves provide potential benefits for human health because of its high content of polyphenols and antioxidant activities with the leaves containing the highest amounts of polyphenols specially catechin with strong antioxidant properties. © 2011 Alam et al; licensee BioMed Central Ltd.
Source Title: BMC Complementary and Alternative Medicine
URI: https://scholarbank.nus.edu.sg/handle/10635/181626
ISSN: 14726882
DOI: 10.1186/1472-6882-11-65
Rights: Attribution 4.0 International
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