Please use this identifier to cite or link to this item: https://doi.org/10.1111/jcmm.12725
Title: iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium
Authors: Ja, K.P.M.M
Miao, Q
Zhen Tee, N.G
Lim, S.Y
Nandihalli, M
J.A. Ramachandra, C
Mehta, A 
Shim, W 
Keywords: integrin
laminin
angiogenesis
animal experiment
animal model
animal tissue
Article
cardiac stem cell
cell transplantation
controlled study
echocardiography
female
flow cytometry
gene expression profiling
heart function
heart infarction
heart infarction size
heart left ventricle ejection fraction
heart muscle cell
heart stroke volume
human
human cell
immunohistochemistry
mouse
neovascularization (pathology)
newborn
nonhuman
pluripotent stem cell
priority journal
real time polymerase chain reaction
telocyte
angiogenesis
animal
cardiac muscle
cardiac muscle cell
cell line
heart infarction
heart ventricle remodeling
induced pluripotent stem cell
pathology
pathophysiology
physiology
SCID mouse
stem cell
Animals
Cell Line
Female
Humans
Induced Pluripotent Stem Cells
Mice
Mice, SCID
Myocardial Infarction
Myocardium
Myocytes, Cardiac
Neovascularization, Physiologic
Stem Cells
Ventricular Remodeling
Issue Date: 2016
Citation: Ja, K.P.M.M, Miao, Q, Zhen Tee, N.G, Lim, S.Y, Nandihalli, M, J.A. Ramachandra, C, Mehta, A, Shim, W (2016). iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium. Journal of Cellular and Molecular Medicine 20 (2) : 323-332. ScholarBank@NUS Repository. https://doi.org/10.1111/jcmm.12725
Rights: Attribution 4.0 International
Abstract: We investigate the effects of myocardial transplantation of human induced pluripotent stem cell (iPSC)-derived progenitors and cardiomyocytes into acutely infarcted myocardium in severe combined immune deficiency mice. A total of 2 × 105 progenitors, cardiomyocytes or cell-free saline were injected into peri-infarcted anterior free wall. Sham-operated animals received no injection. Myocardial function was assessed at 2-week and 4-week post-infarction by using echocardiography and pressure-volume catheterization. Early myocardial remodelling was observed at 2-week with echocardiography derived stroke volume (SV) in saline (20.45 ± 7.36 μl, P < 0.05) and cardiomyocyte (19.52 ± 3.97 μl, P < 0.05) groups, but not in progenitor group (25.65 ± 3.61 μl), significantly deteriorated as compared to sham control group (28.41 ± 4.41 μl). Consistently, pressure-volume haemodynamic measurements showed worsening chamber dilation in saline (EDV: 23.24 ± 5.01 μl, P < 0.05; ESV: 17.08 ± 5.82 μl, P < 0.05) and cardiomyocyte (EDV: 26.45 ± 5.69 μl, P < 0.05; ESV: 18.03 ± 6.58 μl, P < 0.05) groups by 4-week post-infarction as compared to control (EDV: 15.26 ± 2.96 μl; ESV: 8.41 ± 2.94 μl). In contrast, cardiac progenitors (EDV: 20.09 ± 7.76 μl; ESV: 13.98 ± 6.74 μl) persistently protected chamber geometry against negative cardiac remodelling. Similarly, as compared to sham control (54.64 ± 11.37%), LV ejection fraction was preserved in progenitor group from 2-(38.68 ± 7.34%) to 4-week (39.56 ± 13.26%) while cardiomyocyte (36.52 ± 11.39%, P < 0.05) and saline (35.34 ± 11.86%, P < 0.05) groups deteriorated early at 2-week. Improvements of myocardial function in the progenitor group corresponded to increased vascularization (16.12 ± 1.49/mm2 to 25.48 ± 2.08/mm2 myocardial tissue, P < 0.05) and coincided with augmented networking of cardiac telocytes in the interstitial space of infarcted zone. © 2016 John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
Source Title: Journal of Cellular and Molecular Medicine
URI: https://scholarbank.nus.edu.sg/handle/10635/181394
ISSN: 15821838
DOI: 10.1111/jcmm.12725
Rights: Attribution 4.0 International
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