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https://doi.org/10.1111/jcmm.12725
Title: | iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium | Authors: | Ja, K.P.M.M Miao, Q Zhen Tee, N.G Lim, S.Y Nandihalli, M J.A. Ramachandra, C Mehta, A Shim, W |
Keywords: | integrin laminin angiogenesis animal experiment animal model animal tissue Article cardiac stem cell cell transplantation controlled study echocardiography female flow cytometry gene expression profiling heart function heart infarction heart infarction size heart left ventricle ejection fraction heart muscle cell heart stroke volume human human cell immunohistochemistry mouse neovascularization (pathology) newborn nonhuman pluripotent stem cell priority journal real time polymerase chain reaction telocyte angiogenesis animal cardiac muscle cardiac muscle cell cell line heart infarction heart ventricle remodeling induced pluripotent stem cell pathology pathophysiology physiology SCID mouse stem cell Animals Cell Line Female Humans Induced Pluripotent Stem Cells Mice Mice, SCID Myocardial Infarction Myocardium Myocytes, Cardiac Neovascularization, Physiologic Stem Cells Ventricular Remodeling |
Issue Date: | 2016 | Citation: | Ja, K.P.M.M, Miao, Q, Zhen Tee, N.G, Lim, S.Y, Nandihalli, M, J.A. Ramachandra, C, Mehta, A, Shim, W (2016). iPSC-derived human cardiac progenitor cells improve ventricular remodelling via angiogenesis and interstitial networking of infarcted myocardium. Journal of Cellular and Molecular Medicine 20 (2) : 323-332. ScholarBank@NUS Repository. https://doi.org/10.1111/jcmm.12725 | Rights: | Attribution 4.0 International | Abstract: | We investigate the effects of myocardial transplantation of human induced pluripotent stem cell (iPSC)-derived progenitors and cardiomyocytes into acutely infarcted myocardium in severe combined immune deficiency mice. A total of 2 × 105 progenitors, cardiomyocytes or cell-free saline were injected into peri-infarcted anterior free wall. Sham-operated animals received no injection. Myocardial function was assessed at 2-week and 4-week post-infarction by using echocardiography and pressure-volume catheterization. Early myocardial remodelling was observed at 2-week with echocardiography derived stroke volume (SV) in saline (20.45 ± 7.36 μl, P < 0.05) and cardiomyocyte (19.52 ± 3.97 μl, P < 0.05) groups, but not in progenitor group (25.65 ± 3.61 μl), significantly deteriorated as compared to sham control group (28.41 ± 4.41 μl). Consistently, pressure-volume haemodynamic measurements showed worsening chamber dilation in saline (EDV: 23.24 ± 5.01 μl, P < 0.05; ESV: 17.08 ± 5.82 μl, P < 0.05) and cardiomyocyte (EDV: 26.45 ± 5.69 μl, P < 0.05; ESV: 18.03 ± 6.58 μl, P < 0.05) groups by 4-week post-infarction as compared to control (EDV: 15.26 ± 2.96 μl; ESV: 8.41 ± 2.94 μl). In contrast, cardiac progenitors (EDV: 20.09 ± 7.76 μl; ESV: 13.98 ± 6.74 μl) persistently protected chamber geometry against negative cardiac remodelling. Similarly, as compared to sham control (54.64 ± 11.37%), LV ejection fraction was preserved in progenitor group from 2-(38.68 ± 7.34%) to 4-week (39.56 ± 13.26%) while cardiomyocyte (36.52 ± 11.39%, P < 0.05) and saline (35.34 ± 11.86%, P < 0.05) groups deteriorated early at 2-week. Improvements of myocardial function in the progenitor group corresponded to increased vascularization (16.12 ± 1.49/mm2 to 25.48 ± 2.08/mm2 myocardial tissue, P < 0.05) and coincided with augmented networking of cardiac telocytes in the interstitial space of infarcted zone. © 2016 John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. | Source Title: | Journal of Cellular and Molecular Medicine | URI: | https://scholarbank.nus.edu.sg/handle/10635/181394 | ISSN: | 15821838 | DOI: | 10.1111/jcmm.12725 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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