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Title: Metabolic consequences of p300 gene deletion in human colon cancer cells
Authors: Bundy, J.G
Iyer, N.G 
Gentile, M.S
Hu, D.-E
Kettunen, M
Maia, A.-T
Thorne, N.P
Brenton, J.D
Caldas, C
Brindle, K.M
Keywords: glutamic acid
protein p300
animal experiment
animal tissue
cancer cell
cell growth
colon cancer
controlled study
culture medium
DNA microarray
gene deletion
gene expression
gene function
human cell
in vitro study
in vivo culture
metabolic regulation
priority journal
proton nuclear magnetic resonance
SCID mouse
transcription initiation
tumor localization
Colonic Neoplasms
Culture Media, Conditioned
Gene Deletion
Gene Expression Profiling
HCT116 Cells
Mice, SCID
Nuclear Magnetic Resonance, Biomolecular
p300-CBP Transcription Factors
Transplantation, Heterologous
Issue Date: 2006
Citation: Bundy, J.G, Iyer, N.G, Gentile, M.S, Hu, D.-E, Kettunen, M, Maia, A.-T, Thorne, N.P, Brenton, J.D, Caldas, C, Brindle, K.M (2006). Metabolic consequences of p300 gene deletion in human colon cancer cells. Cancer Research 66 (15) : 7606-7614. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Metabolite profiling using 1H nuclear magnetic resonance (NMR) spectroscopy was used to investigate the metabolic changes associated with deletion of the gene for the transcriptional coactivator p300 in the human colon carcinoma cell line HCT116. Multivariate statistical methods were used to distinguish between metabolite patterns that were dependent on cell growth conditions and those that were specifically associated with loss of p300 function. In the absence of serum, wild-type cells showed slower growth, which was accompanied by a marked decrease in phosphocholine concentration, which was not observed in otherwise isogenic cell lines lacking p300. In the presence of serum, several metabolites were identified as being significantly different between the two cell types, including glutamate and glutamine, a nicotinamide-related compound and glycerophosphocholine (GPC). However, in the absence of serum, these metabolites, with the exception of GPC, were not significantly different, leading us to conclude that most of these changes were context dependent. Transcript profiling, using DNA microarrays, showed changes in the levels of transcripts for several enzymes involved in choline metabolism, which might explain the change in GPC concentration. Localized in vivo 1H NMR measurements on the tumors formed following s.c. implantation of these cells into mice showed an increase in the intensity of the peak from choline-containing compounds in the p300 tumors. These data show that NMR-based metabolite profiling has sufficient sensitivity to identify the metabolic consequences of p300 gene deletion in tumor cells in vitro and in vivo. ©2006 American Association for Cancer Research.
Source Title: Cancer Research
ISSN: 0008-5472
DOI: 10.1158/0008-5472.CAN-05-2999
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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