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https://doi.org/10.1242/jcs.002931
Title: | Candida albicans hyphal morphogenesis occurs in Sec3p-independent and Sec3p-dependent phases separated by septin ring formation | Authors: | Li, C.-R Lee, R.T.-H Wang, Y.-M Zheng, X.-D Wang, Y |
Keywords: | cell cycle protein exocyst protein Cdc10 protein Cdc11 protein Cdc3p protein Sec3p protein Sec5p protein subunit septin unclassified drug article Candida albicans cell cycle phase cell polarity controlled study exocytosis fungus hyphae gene deletion growth regulation immunoprecipitation morphogenesis nonhuman priority journal protein assembly protein function protein localization protein protein interaction protein structure temperature sensitive mutant Biological Markers Candida albicans cdc42 GTP-Binding Protein Cell Polarity Fungal Proteins Genes, Fungal Hyphae Morphogenesis Secretory Vesicles Sequence Deletion Temperature Candida albicans Mammalia |
Issue Date: | 2007 | Citation: | Li, C.-R, Lee, R.T.-H, Wang, Y.-M, Zheng, X.-D, Wang, Y (2007). Candida albicans hyphal morphogenesis occurs in Sec3p-independent and Sec3p-dependent phases separated by septin ring formation. Journal of Cell Science 120 (11) : 1898-1907. ScholarBank@NUS Repository. https://doi.org/10.1242/jcs.002931 | Rights: | Attribution 4.0 International | Abstract: | The growing tips Candida albicans hyphae are sites of polarized exocytosis. Mammalian septins have been implicated in regulating exocytosis and C. albicans septims are known to localize at hyphal tips, although their function here is unknown. Here, we report that C. albicans cells deleted of the exocyst subunit gene SEC3 can grow normal germ tubes, but are unable to maintain tip growth after assembly of the first septin ring, resulting in isotropic expansion of the tip. Deleting either of the septin genes CDC10 or CDC11 caused Sec3p mislocalization and surprisingly, also restored hyphal development in the sec3? mutant without rescuing the temperature sensitivity. Co-immunoprecipitation experiments detected association of the septin Cdc3p with the exocyst subunits Sec3p and Sec5p. Our results reveal that C. albicans hyphal development occurs through Sec3p-independent and dependent phases, and provide strong genetic and biochemical evidence for a role of septins in polarized exocytosis. | Source Title: | Journal of Cell Science | URI: | https://scholarbank.nus.edu.sg/handle/10635/181037 | ISSN: | 0021-9533 | DOI: | 10.1242/jcs.002931 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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