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Title: Candida albicans hyphal morphogenesis occurs in Sec3p-independent and Sec3p-dependent phases separated by septin ring formation
Authors: Li, C.-R
Lee, R.T.-H
Wang, Y.-M
Zheng, X.-D
Wang, Y 
Keywords: cell cycle protein
protein Cdc10
protein Cdc11
protein Cdc3p
protein Sec3p
protein Sec5p
protein subunit
unclassified drug
Candida albicans
cell cycle phase
cell polarity
controlled study
fungus hyphae
gene deletion
growth regulation
priority journal
protein assembly
protein function
protein localization
protein protein interaction
protein structure
temperature sensitive mutant
Biological Markers
Candida albicans
cdc42 GTP-Binding Protein
Cell Polarity
Fungal Proteins
Genes, Fungal
Secretory Vesicles
Sequence Deletion
Candida albicans
Issue Date: 2007
Citation: Li, C.-R, Lee, R.T.-H, Wang, Y.-M, Zheng, X.-D, Wang, Y (2007). Candida albicans hyphal morphogenesis occurs in Sec3p-independent and Sec3p-dependent phases separated by septin ring formation. Journal of Cell Science 120 (11) : 1898-1907. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The growing tips Candida albicans hyphae are sites of polarized exocytosis. Mammalian septins have been implicated in regulating exocytosis and C. albicans septims are known to localize at hyphal tips, although their function here is unknown. Here, we report that C. albicans cells deleted of the exocyst subunit gene SEC3 can grow normal germ tubes, but are unable to maintain tip growth after assembly of the first septin ring, resulting in isotropic expansion of the tip. Deleting either of the septin genes CDC10 or CDC11 caused Sec3p mislocalization and surprisingly, also restored hyphal development in the sec3? mutant without rescuing the temperature sensitivity. Co-immunoprecipitation experiments detected association of the septin Cdc3p with the exocyst subunits Sec3p and Sec5p. Our results reveal that C. albicans hyphal development occurs through Sec3p-independent and dependent phases, and provide strong genetic and biochemical evidence for a role of septins in polarized exocytosis.
Source Title: Journal of Cell Science
ISSN: 0021-9533
DOI: 10.1242/jcs.002931
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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