Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms7553
Title: Mammalian adaptation of influenza A(H7N9) virus is limited by a narrow genetic bottleneck
Authors: Zaraket, H
Baranovich, T
Kaplan, B.S
Carter, R
Song, M.-S
Paulson, J.C
Rehg, J.E
Bahl, J 
Crumpton, J.C
Seiler, J
Edmonson, M
Wu, G
Karlsson, E
Fabrizio, T
Zhu, H
Guan, Y
Husain, M
Schultz-Cherry, S
Krauss, S
McBride, R
Webster, R.G
Govorkova, E.A
Zhang, J
Russell, C.J
Webby, R.J
Keywords: adaptation
avian influenza
disease transmission
enzyme activity
epidemic
fitness
mammal
mutation
population bottleneck
poultry
public health
risk assessment
virus
adaptation
adult
amino acid substitution
animal cell
animal experiment
animal model
Article
chicken
controlled study
ferret
gene replication
genetic analysis
genetic variability
human
human cell
in vitro study
Influenza virus A H7N9
male
MDCK cell line
neutrophil chemotaxis
nonhuman
receptor binding
risk assessment
sequence analysis
virus infectivity
virus recombinant
virus shedding
virus transmission
virus virulence
young adult
adaptation
animal
asymptomatic infection
avian influenza
Chlorocebus aethiops
dog
genetic variation
genetics
HEK293 cell line
influenza
Influenza A virus (H7N9)
multiplex polymerase chain reaction
mutation
orthomyxovirus infection
pathogenicity
reverse transcription polymerase chain reaction
transmission
Vero cell line
virology
Aves
Gallus gallus
Influenza A virus
Mammalia
Mustela
Orthomyxoviridae
core protein
influenza virus polymerase basic protein 1
NA protein, influenza A virus
NP protein, Influenza A virus
RNA binding protein
sialidase
viral protein
virus RNA
Adaptation, Physiological
Animals
Asymptomatic Infections
Cercopithecus aethiops
Chickens
Dogs
Ferrets
Genetic Variation
HEK293 Cells
Humans
Influenza A Virus, H7N9 Subtype
Influenza in Birds
Influenza, Human
Madin Darby Canine Kidney Cells
Multiplex Polymerase Chain Reaction
Mutation
Neuraminidase
Orthomyxoviridae Infections
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral
RNA-Binding Proteins
Vero Cells
Viral Core Proteins
Viral Proteins
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Zaraket, H, Baranovich, T, Kaplan, B.S, Carter, R, Song, M.-S, Paulson, J.C, Rehg, J.E, Bahl, J, Crumpton, J.C, Seiler, J, Edmonson, M, Wu, G, Karlsson, E, Fabrizio, T, Zhu, H, Guan, Y, Husain, M, Schultz-Cherry, S, Krauss, S, McBride, R, Webster, R.G, Govorkova, E.A, Zhang, J, Russell, C.J, Webby, R.J (2015). Mammalian adaptation of influenza A(H7N9) virus is limited by a narrow genetic bottleneck. Nature Communications 6 : 6553. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms7553
Rights: Attribution 4.0 International
Abstract: Human infection with avian influenza A(H7N9) virus is associated mainly with the exposure to infected poultry. The factors that allow interspecies transmission but limit human-to-human transmission are unknown. Here we show that A/Anhui/1/2013(H7N9) influenza virus infection of chickens (natural hosts) is asymptomatic and that it generates a high genetic diversity. In contrast, diversity is tightly restricted in infected ferrets, limiting further adaptation to a fully transmissible form. Airborne transmission in ferrets is accompanied by the mutations in PB1, NP and NA genes that reduce viral polymerase and neuraminidase activity. Therefore, while A(H7N9) virus can infect mammals, further adaptation appears to incur a fitness cost. Our results reveal that a tight genetic bottleneck during avian-to-mammalian transmission is a limiting factor in A(H7N9) influenza virus adaptation to mammals. This previously unrecognized biological mechanism limiting species jumps provides a measure of adaptive potential and may serve as a risk assessment tool for pandemic preparedness. © 2015 Macmillan Publishers Limited. All rights reserved.
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/180476
ISSN: 2041-1723
DOI: 10.1038/ncomms7553
Rights: Attribution 4.0 International
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