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https://doi.org/10.1038/ncomms8203
| Title: | Developmental-stage-dependent transcriptional response to leukaemic oncogene expression | Authors: | Regha, K Assi, S.A Tsoulaki, O Gilmour, J Lacaud, G Bonifer, C |
Keywords: | hybrid protein RUNX1 ETO protein transcription factor RUNX1 unclassified drug DNA binding protein messenger RNA MTG8 protein, mouse oncoprotein Runx1 protein, mouse transcription factor transcription factor RUNX1 transcriptome blood developmental stage gene expression genetic differentiation germ cell molecular analysis acute myeloblastic leukemia animal cell Article binding site blood cell cell differentiation cell expansion cell population cell selection chromatin immunoprecipitation controlled study developmental stage down regulation embryo embryonic stem cell flow cytometry gene expression gene repression genetic transcription hemangioblast hematopoietic cell hematopoietic stem cell human human cell in vitro study leukemogenesis mouse multipotent stem cell myeloid progenitor cell myelopoiesis nonhuman oncogene phenotype protein binding protein induction target cell upregulation acute myeloid leukemia animal cell culture technique cytology electroporation gene expression regulation gene regulatory network gene translocation genetics hematopoiesis metabolism mouse embryonic stem cell Western blotting Animals Blotting, Western Cell Culture Techniques Chromatin Immunoprecipitation Core Binding Factor Alpha 2 Subunit DNA-Binding Proteins Electroporation Flow Cytometry Gene Expression Regulation, Developmental Gene Expression Regulation, Neoplastic Gene Regulatory Networks Hematopoiesis In Vitro Techniques Leukemia, Myeloid, Acute Mice Mouse Embryonic Stem Cells Oncogene Proteins, Fusion Proto-Oncogene Proteins RNA, Messenger Transcription Factors Transcriptome Translocation, Genetic |
Issue Date: | 2015 | Publisher: | Nature Publishing Group | Citation: | Regha, K, Assi, S.A, Tsoulaki, O, Gilmour, J, Lacaud, G, Bonifer, C (2015). Developmental-stage-dependent transcriptional response to leukaemic oncogene expression. Nature Communications 6 : 7203. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms8203 | Rights: | Attribution 4.0 International | Abstract: | Acute myeloid leukaemia (AML) is characterized by a block in myeloid differentiation the stage of which is dependent on the nature of the transforming oncogene and the developmental stage of the oncogenic hit. This is also true for the t(8;21) translocation that gives rise to the RUNX1-ETO fusion protein and initiates the most common form of human AML. Here we study the differentiation of mouse embryonic stem cells expressing an inducible RUNX1-ETO gene into blood cells as a model, combined with genome-wide analyses of transcription factor binding and gene expression. RUNX1-ETO interferes with both the activating and repressive function of its normal counterpart, RUNX1, at early and late stages of blood cell development. However, the response of the transcriptional network to RUNX1-ETO expression is developmental stage specific, highlighting the molecular mechanisms determining specific target cell expansion after an oncogenic hit. © 2015 Macmillan Publishers Limited. All rights reserved. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/180469 | ISSN: | 2041-1723 | DOI: | 10.1038/ncomms8203 | Rights: | Attribution 4.0 International |
| Appears in Collections: | Elements Staff Publications |
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