Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms9218
Title: Nuclear envelope-associated endosomes deliver surface proteins to the nucleus
Authors: Chaumet, A
Wright, G.D
Seet, S.H
Tham, K.M
Gounko, N.V
Bard, F 
Keywords: cell surface protein
early endosome antigen 1
epidermal growth factor receptor
histone H1
membrane protein
nuclear protein
protein disulfide isomerase
Pseudomonas exotoxin
SUN1 protein
SUN2 protein
translocon
tubulin
unclassified drug
bacterial toxin
exotoxin
membrane protein
microtubule associated protein
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
nuclear localization signal
nuclear protein
SEC61A1 protein, human
signal peptide
SUN1 protein, human
SUN2 protein, human
toxA protein, Pseudomonas aeruginosa
translocon
virulence factor
bacterium
cell organelle
cells and cell components
mobility
protein
RNA
translocation
Article
cell fractionation
cell nucleus
cell nucleus membrane
cell surface
controlled study
cytolysis
cytosol
cytosolic fraction
electron microscopy
endosome
female
Golgi complex
HeLa cell line
human
human cell
immunoprecipitation
inner membrane
nuclear import
nuclear localization signal
nuclear pore
outer membrane
protein binding
protein localization
protein transport
regulatory mechanism
RNA interference
Western blotting
cell nucleus
cell nucleus membrane
confocal microscopy
endocytosis
endoplasmic reticulum
endosome
fluorescent antibody technique
gene silencing
genetics
metabolism
tumor cell line
Bacteria (microorganisms)
Pseudomonas exotoxin
ADP Ribose Transferases
Bacterial Toxins
Cell Line, Tumor
Cell Nucleus
Cytosol
Endocytosis
Endoplasmic Reticulum
Endosomes
Exotoxins
Fluorescent Antibody Technique
Gene Knockdown Techniques
Golgi Apparatus
HeLa Cells
Humans
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Microscopy, Confocal
Microscopy, Electron
Microtubule-Associated Proteins
Nuclear Envelope
Nuclear Localization Signals
Nuclear Proteins
Protein Transport
SEC Translocation Channels
Virulence Factors
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Chaumet, A, Wright, G.D, Seet, S.H, Tham, K.M, Gounko, N.V, Bard, F (2015). Nuclear envelope-associated endosomes deliver surface proteins to the nucleus. Nature Communications 6 : 8218. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms9218
Rights: Attribution 4.0 International
Abstract: Endocytosis directs molecular cargo along three main routes: recycling to the cell surface, transport to the Golgi apparatus or degradation in endolysosomes. Pseudomonas exotoxin A (PE) is a bacterial protein that typically traffics to the Golgi and then the endoplasmic reticulum before translocating to the cytosol. Here we show that a substantial fraction of internalized PE is also located in nuclear envelope-associated endosomes (NAE), which display limited mobility, exhibit a propensity to undergo fusion and readily discharge their contents into the nuclear envelope. Electron microscopy and protein trapping in the nucleus indicate that NAE mediate PE transfer into the nucleoplasm. RNAi screening further revealed that NAE-mediated transfer depends on the nuclear envelope proteins SUN1 and SUN2, as well as the Sec61 translocon complex. These data reveal a novel endosomal route from the cell surface to the nucleoplasm that facilitates the accumulation of extracellular and cell surface proteins in the nucleus. © 2015 Macmillan Publishers Limited. All rights reserved.
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/180439
ISSN: 2041-1723
DOI: 10.1038/ncomms9218
Rights: Attribution 4.0 International
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