Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/180286
Title: Correlating confocal microscopy and atomic force indentation reveals metastatic cancer cells stiffen during invasion into collagen i matrices
Authors: Staunton, J.R
Doss, B.L 
Lindsay, S
Ros, R
Keywords: collagen type 1
algorithm
atomic force microscopy
confocal microscopy
extracellular matrix
human
mechanics
metabolism
metastasis
neoplasm
pathology
theoretical model
tumor cell line
Young modulus
Algorithms
Cell Line, Tumor
Collagen Type I
Elastic Modulus
Extracellular Matrix
Humans
Mechanical Phenomena
Microscopy, Atomic Force
Microscopy, Confocal
Models, Theoretical
Neoplasm Metastasis
Neoplasms
Issue Date: 2016
Citation: Staunton, J.R, Doss, B.L, Lindsay, S, Ros, R (2016). Correlating confocal microscopy and atomic force indentation reveals metastatic cancer cells stiffen during invasion into collagen i matrices. Scientific Reports 6 : 19686. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Mechanical interactions between cells and their microenvironment dictate cell phenotype and behavior, calling for cell mechanics measurements in three-dimensional (3D) extracellular matrices (ECM). Here we describe a novel technique for quantitative mechanical characterization of soft, heterogeneous samples in 3D. The technique is based on the integration of atomic force microscopy (AFM) based deep indentation, confocal fluorescence microscopy, finite element (FE) simulations and analytical modeling. With this method, the force response of a cell embedded in 3D ECM can be decoupled from that of its surroundings, enabling quantitative determination of the elastic properties of both the cell and the matrix. We applied the technique to the quantification of the elastic properties of metastatic breast adenocarcinoma cells invading into collagen hydrogels. We found that actively invading and fully embedded cells are significantly stiffer than cells remaining on top of the collagen, a clear example of phenotypical change in response to the 3D environment. Treatment with Rho-associated protein kinase (ROCK) inhibitor significantly reduces this stiffening, indicating that actomyosin contractility plays a major role in the initial steps of metastatic invasion.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/180286
ISSN: 20452322
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_srep19686.pdf4.71 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

7
checked on Nov 26, 2020

Google ScholarTM

Check


This item is licensed under a Creative Commons License Creative Commons