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Title: Propiece IL-1? facilitates the growth of acute T-lymphocytic leukemia cells through the activation of NF-?B and SP1
Authors: Zhang, Y
Yu, X
Lin, D
Lei, L
Hu, B
Cao, F
Mei, Y 
Wu, D
Liu, H 
Keywords: cisplatin
immunoglobulin enhancer binding protein
propiece interleukin 1alpha
transcription factor Sp1
unclassified drug
IL1A protein, human
immunoglobulin enhancer binding protein
interleukin 1alpha
protein binding
transcription factor Sp1
acute lymphoblastic leukemia
animal cell
animal experiment
animal model
cancer growth
cell proliferation
controlled study
drug resistance
gene expression
human cell
in vivo study
promoter region
protein expression
acute lymphoblastic leukemia
Bagg albino mouse
cell nucleus
gene expression regulation
HEK293 cell line
Jurkat cell line
nucleotide sequence
nude mouse
reverse transcription polymerase chain reaction
tumor cell line
Western blotting
Base Sequence
Blotting, Western
Cell Line, Tumor
Cell Nucleus
Cell Proliferation
Gene Expression Regulation, Leukemic
HEK293 Cells
Jurkat Cells
Mice, Inbred BALB C
Mice, Nude
NF-kappa B
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Promoter Regions, Genetic
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Sp1 Transcription Factor
Transplantation, Heterologous
Issue Date: 2017
Publisher: Impact Journals LLC
Citation: Zhang, Y, Yu, X, Lin, D, Lei, L, Hu, B, Cao, F, Mei, Y, Wu, D, Liu, H (2017). Propiece IL-1? facilitates the growth of acute T-lymphocytic leukemia cells through the activation of NF-?B and SP1. Oncotarget 8 (9) : 15677-15688. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Interleukin 1? (IL-1?) is a pro-inflammatory cytokine that possesses multiple immune-regulatory functions. It is mainly expressed as the cell-associated form and not actively secreted in healthy tissues. The intracellular IL-1? has been shown to be a chromatin-associated cytokine and can affect transcription. There are spontaneous expressions of IL-1? in acute lymphocytic leukemia (ALL) blasts. However, the role of nuclear-localized IL-1? in ALL is not clear. Here we showed that overexpression of the nuclear form of IL-1? (propiece IL-1?) could promote proliferation and reduce apoptosis of T-ALL cells. It also increased the ALL cells' resistance to low serum concentration and cisplatin treatment. In vivo growth of the T-ALL cells overexpressing the propiece IL-1? were also enhanced compared to the control cells. Microarray analysis revealed many changes in gene expressions related to cell growth and stress, including a group of metallothionein genes. Moreover, the expressions of transcription factors, NF?B and specific protein 1 (SP1), were up-regulated by propiece IL-1?. Propiece IL-1? could bind to the promoter of SP1 and a binding sequence logo was identified. Therefore, nuclear expression of propiece IL-1? can facilitate the growth of T-ALL cells possibly through the activation of NF?B and SP1.
Source Title: Oncotarget
ISSN: 1949-2553
DOI: 10.18632/oncotarget.14934
Rights: Attribution 4.0 International
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