Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep41419
Title: Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol
Authors: Wang, J
Zhang, L 
Huang, Y
Dandapat, A
Dai, L
Zhang, G
Lu, X
Zhang, J
Lai, W
Chen, T
Keywords: clenbuterol
gold
metal nanoparticle
silver
chemistry
color
immunoaffinity chromatography
limit of detection
porosity
procedures
staining
surface property
ultrastructure
ultraviolet spectrophotometry
Clenbuterol
Color
Gold
Immunochromatography
Limit of Detection
Metal Nanoparticles
Porosity
Silver
Spectrophotometry, Ultraviolet
Staining and Labeling
Surface Properties
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Wang, J, Zhang, L, Huang, Y, Dandapat, A, Dai, L, Zhang, G, Lu, X, Zhang, J, Lai, W, Chen, T (2017). Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol. Scientific Reports 7 : 41419. ScholarBank@NUS Repository. https://doi.org/10.1038/srep41419
Rights: Attribution 4.0 International
Abstract: The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip (ICTS). Unlike conventional ICTS assay usually uses single-component, solid gold nanoparticles as labeled probes, in our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS was successfully developed for the detection of clenbuterol (CLE). The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were synthesized by varying the volume ratio of [HAuCl4]:[Ag NPs] via the galvanic replacement reaction. The surface of hollow Ag-Au NPs was functionalized with 11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE monoclonal antibody. Overall size of the Au-Ag NPs, size of the holes within individual NPs and also Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection signals and the quantitative data. The sensitivity of optimized hollow Au-Ag NPs probes has been achieved even as low as 2 ppb in a short time (within 15 min), which is superior over the detection performance of conventional test strip using Au NPs. The optimized hollow Au-Ag NPs labeled test strip can be used as an ideal candidate for the rapid screening of CLE in food samples. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/179739
ISSN: 2045-2322
DOI: 10.1038/srep41419
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_srep41419.pdf2.63 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

26
checked on Jul 18, 2021

Page view(s)

72
checked on Jul 22, 2021

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons