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Title: Coordination of chromosome segregation and cell division in Staphylococcus aureus
Authors: Bottomley, A.L
Liew, A.T.F 
Kusuma, K.D
Peterson, E
Seidel, L
Foster, S.J
Harry, E.J
Keywords: FtsZ protein
protein DnaK
Bacillus subtilis
cell division
cell viability
chromosome segregation
controlled study
fluorescence microscopy
growth rate
liquid chromatography-mass spectrometry
polyacrylamide gel electrophoresis
polymerase chain reaction
protein purification
Staphylococcus aureus
Western blotting
Issue Date: 2017
Citation: Bottomley, A.L, Liew, A.T.F, Kusuma, K.D, Peterson, E, Seidel, L, Foster, S.J, Harry, E.J (2017). Coordination of chromosome segregation and cell division in Staphylococcus aureus. Frontiers in Microbiology 8 (AUG) : 1575. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Productive bacterial cell division and survival of progeny requires tight coordination between chromosome segregation and cell division to ensure equal partitioning of DNA. Unlike rod-shaped bacteria that undergo division in one plane, the coccoid human pathogen Staphylococcus aureus divides in three successive orthogonal planes, which requires a different spatial control compared to rod-shaped cells. To gain a better understanding of how this coordination between chromosome segregation and cell division is regulated in S. aureus, we investigated proteins that associate with FtsZ and the divisome. We found that DnaK, a well-known chaperone, interacts with FtsZ, EzrA and DivIVA, and is required for DivIVA stability. Unlike in several rod shaped organisms, DivIVA in S. aureus associates with several components of the divisome, as well as the chromosome segregation protein, SMC. This data, combined with phenotypic analysis of mutants, suggests a novel role for S. aureus DivIVA in ensuring cell division and chromosome segregation are coordinated. @ 2017 Bottomley, Liew, Kusuma, Peterson, Seidel, Foster and Harry.
Source Title: Frontiers in Microbiology
ISSN: 1664302X
DOI: 10.3389/fmicb.2017.01575
Rights: Attribution 4.0 International
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