Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmicb.2017.01575
Title: Coordination of chromosome segregation and cell division in Staphylococcus aureus
Authors: Bottomley, A.L
Liew, A.T.F 
Kusuma, K.D
Peterson, E
Seidel, L
Foster, S.J
Harry, E.J
Keywords: FtsZ protein
protein DnaK
Article
Bacillus subtilis
cell division
cell viability
chromosome segregation
controlled study
cytolysis
electroporation
fluorescence microscopy
growth rate
immunoblotting
liquid chromatography-mass spectrometry
microscopy
nonhuman
phenotype
polyacrylamide gel electrophoresis
polymerase chain reaction
protein purification
Staphylococcus aureus
Western blotting
Issue Date: 2017
Citation: Bottomley, A.L, Liew, A.T.F, Kusuma, K.D, Peterson, E, Seidel, L, Foster, S.J, Harry, E.J (2017). Coordination of chromosome segregation and cell division in Staphylococcus aureus. Frontiers in Microbiology 8 (AUG) : 1575. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2017.01575
Rights: Attribution 4.0 International
Abstract: Productive bacterial cell division and survival of progeny requires tight coordination between chromosome segregation and cell division to ensure equal partitioning of DNA. Unlike rod-shaped bacteria that undergo division in one plane, the coccoid human pathogen Staphylococcus aureus divides in three successive orthogonal planes, which requires a different spatial control compared to rod-shaped cells. To gain a better understanding of how this coordination between chromosome segregation and cell division is regulated in S. aureus, we investigated proteins that associate with FtsZ and the divisome. We found that DnaK, a well-known chaperone, interacts with FtsZ, EzrA and DivIVA, and is required for DivIVA stability. Unlike in several rod shaped organisms, DivIVA in S. aureus associates with several components of the divisome, as well as the chromosome segregation protein, SMC. This data, combined with phenotypic analysis of mutants, suggests a novel role for S. aureus DivIVA in ensuring cell division and chromosome segregation are coordinated. @ 2017 Bottomley, Liew, Kusuma, Peterson, Seidel, Foster and Harry.
Source Title: Frontiers in Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/179463
ISSN: 1664302X
DOI: 10.3389/fmicb.2017.01575
Rights: Attribution 4.0 International
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