Please use this identifier to cite or link to this item: https://doi.org/10.1155/2017/9653194
Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs
Authors: Reiner, A.T
Tan, S
Agreiter, C
Auer, K
Bachmayr-Heyda, A
Aust, S
Pecha, N
Mandorfer, M
Pils, D
Brisson, A.R
Zeillinger, R
Lim, S.K 
Keywords: gelatinase A
gelatinase B
lipid binding protein
lipocortin 5
gelatinase B
lipocortin 5
MMP9 protein, human
protein binding
tumor marker
adult
aged
Article
ascites
cancer grading
cancer localization
cancer patient
cancer tissue
clinical article
controlled study
cryoelectron microscopy
cytoreductive surgery
exosome
female
high grade serous ovarian cancer
histology
human
human tissue
liver cirrhosis
malignant ascites
ovary cancer
portal hypertension
protein binding
signal noise ratio
zymography
ascites
carcinosarcoma
case control study
exosome
metabolism
middle aged
ovary tumor
pathology
Aged
Annexin A5
Ascites
Biomarkers, Tumor
Carcinosarcoma
Case-Control Studies
Extracellular Vesicles
Female
Humans
Matrix Metalloproteinase 9
Middle Aged
Ovarian Neoplasms
Protein Binding
Issue Date: 2017
Publisher: Hindawi Limited
Citation: Reiner, A.T, Tan, S, Agreiter, C, Auer, K, Bachmayr-Heyda, A, Aust, S, Pecha, N, Mandorfer, M, Pils, D, Brisson, A.R, Zeillinger, R, Lim, S.K (2017). EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs. Disease Markers 2017 : 9653194. ScholarBank@NUS Repository. https://doi.org/10.1155/2017/9653194
Rights: Attribution 4.0 International
Abstract: High-grade serous ovarian cancer (HGSOC) is the most aggressive type of ovarian cancer and is responsible for most deaths caused by gynecological cancers. Numerous candidate biomarkers were identified for this disease in the last decades, but most were not sensitive or specific enough for clinical applications. Hence, new biomarkers for HGSOC are urgently required. This study aimed to identify new markers by isolating different extracellular vesicle (EV) types from the ascites of ovarian cancer patients according to their affinities for lipid-binding proteins and analyzing their protein cargo. This approach circumvents the low signal-To-noise ratio when using biological fluids for biomarker discovery and the issue of contamination by large non-EV complexes. We isolated and analyzed three distinct EV populations from the ascites of patients with ovarian cancer or cirrhosis and observed that Annexin V-binding EVs have higher levels of matrix metalloproteinase 9 in malignant compared to portal-hypertensive ascites. As this protein was not detected in other EV populations, this study validates our approach of using different EV types for optimal biomarker discovery. Furthermore, MMP9 in Annexin V-binding EVs could be a HGSOC biomarker with enhanced specificity, because its identification requires detection of two distinct components, that is, lipid and protein. © 2017 Agnes T. Reiner et al.
Source Title: Disease Markers
URI: https://scholarbank.nus.edu.sg/handle/10635/179263
ISSN: 0278-0240
DOI: 10.1155/2017/9653194
Rights: Attribution 4.0 International
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