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https://doi.org/10.1155/2017/9653194
Title: | EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs | Authors: | Reiner, A.T Tan, S Agreiter, C Auer, K Bachmayr-Heyda, A Aust, S Pecha, N Mandorfer, M Pils, D Brisson, A.R Zeillinger, R Lim, S.K |
Keywords: | gelatinase A gelatinase B lipid binding protein lipocortin 5 gelatinase B lipocortin 5 MMP9 protein, human protein binding tumor marker adult aged Article ascites cancer grading cancer localization cancer patient cancer tissue clinical article controlled study cryoelectron microscopy cytoreductive surgery exosome female high grade serous ovarian cancer histology human human tissue liver cirrhosis malignant ascites ovary cancer portal hypertension protein binding signal noise ratio zymography ascites carcinosarcoma case control study exosome metabolism middle aged ovary tumor pathology Aged Annexin A5 Ascites Biomarkers, Tumor Carcinosarcoma Case-Control Studies Extracellular Vesicles Female Humans Matrix Metalloproteinase 9 Middle Aged Ovarian Neoplasms Protein Binding |
Issue Date: | 2017 | Publisher: | Hindawi Limited | Citation: | Reiner, A.T, Tan, S, Agreiter, C, Auer, K, Bachmayr-Heyda, A, Aust, S, Pecha, N, Mandorfer, M, Pils, D, Brisson, A.R, Zeillinger, R, Lim, S.K (2017). EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs. Disease Markers 2017 : 9653194. ScholarBank@NUS Repository. https://doi.org/10.1155/2017/9653194 | Rights: | Attribution 4.0 International | Abstract: | High-grade serous ovarian cancer (HGSOC) is the most aggressive type of ovarian cancer and is responsible for most deaths caused by gynecological cancers. Numerous candidate biomarkers were identified for this disease in the last decades, but most were not sensitive or specific enough for clinical applications. Hence, new biomarkers for HGSOC are urgently required. This study aimed to identify new markers by isolating different extracellular vesicle (EV) types from the ascites of ovarian cancer patients according to their affinities for lipid-binding proteins and analyzing their protein cargo. This approach circumvents the low signal-To-noise ratio when using biological fluids for biomarker discovery and the issue of contamination by large non-EV complexes. We isolated and analyzed three distinct EV populations from the ascites of patients with ovarian cancer or cirrhosis and observed that Annexin V-binding EVs have higher levels of matrix metalloproteinase 9 in malignant compared to portal-hypertensive ascites. As this protein was not detected in other EV populations, this study validates our approach of using different EV types for optimal biomarker discovery. Furthermore, MMP9 in Annexin V-binding EVs could be a HGSOC biomarker with enhanced specificity, because its identification requires detection of two distinct components, that is, lipid and protein. © 2017 Agnes T. Reiner et al. | Source Title: | Disease Markers | URI: | https://scholarbank.nus.edu.sg/handle/10635/179263 | ISSN: | 0278-0240 | DOI: | 10.1155/2017/9653194 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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