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https://doi.org/10.1155/2017/3164375
Title: | Molecular Responses of Human Retinal Cells to Infection with Dengue Virus | Authors: | Carr, J.M Ashander, L.M Calvert, J.K Ma, Y Aloia, A Bracho, G.G Chee, S.-P Appukuttan, B Smith, J.R |
Keywords: | actin antivirus agent beta catenin catenin beta 1 interferon protein ZO1 unclassified drug vascular adhesion protein 1 Article cell function cytopathogenic effect dengue Dengue virus Dengue virus 2 gene overexpression human human cell macular edema protein expression retina cell retinopathy virus replication cell culture cell line cytology Dengue virus endothelium cell epithelium cell immunohistochemistry immunology metabolism pathogenicity physiology retina tight junction virology Cell Line Cells, Cultured Dengue Virus Endothelial Cells Epithelial Cells Humans Immunohistochemistry Retina Tight Junctions Virus Replication |
Issue Date: | 2017 | Publisher: | Hindawi Limited | Citation: | Carr, J.M, Ashander, L.M, Calvert, J.K, Ma, Y, Aloia, A, Bracho, G.G, Chee, S.-P, Appukuttan, B, Smith, J.R (2017). Molecular Responses of Human Retinal Cells to Infection with Dengue Virus. Mediators of Inflammation 2017 : 3164375. ScholarBank@NUS Repository. https://doi.org/10.1155/2017/3164375 | Rights: | Attribution 4.0 International | Abstract: | Recent clinical reports indicate that infection with dengue virus (DENV) commonly has ocular manifestations. The most serious threat to vision is dengue retinopathy, including retinal vasculopathy and macular edema. Mechanisms of retinopathy are unstudied, but observations in patients implicate retinal pigment epithelial cells and retinal endothelial cells. Human retinal cells were inoculated with DENV-2 and monitored for up to 72 hours. Epithelial and endothelial cells supported DENV replication and release, but epithelial cells alone demonstrated clear cytopathic effect, and infection was more productive in those cells. Infection induced type I interferon responses from both cells, but this was stronger in epithelial cells. Endothelial cells increased expression of adhesion molecules, with sustained overexpression of vascular adhesion molecule-1. Transcellular impedance decreased for epithelial monolayers, but not endothelial monolayers, coinciding with cytopathic effect. This reduction was accompanied by disorganization of intracellular filamentous-actin and decreased expression of junctional molecules, zonula occludens 1, and catenin-?1. Changes in endothelial expression of adhesion molecules are consistent with the retinal vasculopathy seen in patients infected with DENV; decreases in epithelial junctional protein expression, paralleling loss of integrity of the epithelium, provide a molecular basis for DENV-associated macular edema. These molecular processes present potential therapeutic targets for vision-threatening dengue retinopathy. © 2017 Jillian M. Carr et al. | Source Title: | Mediators of Inflammation | URI: | https://scholarbank.nus.edu.sg/handle/10635/179251 | ISSN: | 0962-9351 | DOI: | 10.1155/2017/3164375 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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