Please use this identifier to cite or link to this item:
https://doi.org/10.1155/2017/3164375
DC Field | Value | |
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dc.title | Molecular Responses of Human Retinal Cells to Infection with Dengue Virus | |
dc.contributor.author | Carr, J.M | |
dc.contributor.author | Ashander, L.M | |
dc.contributor.author | Calvert, J.K | |
dc.contributor.author | Ma, Y | |
dc.contributor.author | Aloia, A | |
dc.contributor.author | Bracho, G.G | |
dc.contributor.author | Chee, S.-P | |
dc.contributor.author | Appukuttan, B | |
dc.contributor.author | Smith, J.R | |
dc.date.accessioned | 2020-10-23T02:37:19Z | |
dc.date.available | 2020-10-23T02:37:19Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Carr, J.M, Ashander, L.M, Calvert, J.K, Ma, Y, Aloia, A, Bracho, G.G, Chee, S.-P, Appukuttan, B, Smith, J.R (2017). Molecular Responses of Human Retinal Cells to Infection with Dengue Virus. Mediators of Inflammation 2017 : 3164375. ScholarBank@NUS Repository. https://doi.org/10.1155/2017/3164375 | |
dc.identifier.issn | 0962-9351 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/179251 | |
dc.description.abstract | Recent clinical reports indicate that infection with dengue virus (DENV) commonly has ocular manifestations. The most serious threat to vision is dengue retinopathy, including retinal vasculopathy and macular edema. Mechanisms of retinopathy are unstudied, but observations in patients implicate retinal pigment epithelial cells and retinal endothelial cells. Human retinal cells were inoculated with DENV-2 and monitored for up to 72 hours. Epithelial and endothelial cells supported DENV replication and release, but epithelial cells alone demonstrated clear cytopathic effect, and infection was more productive in those cells. Infection induced type I interferon responses from both cells, but this was stronger in epithelial cells. Endothelial cells increased expression of adhesion molecules, with sustained overexpression of vascular adhesion molecule-1. Transcellular impedance decreased for epithelial monolayers, but not endothelial monolayers, coinciding with cytopathic effect. This reduction was accompanied by disorganization of intracellular filamentous-actin and decreased expression of junctional molecules, zonula occludens 1, and catenin-?1. Changes in endothelial expression of adhesion molecules are consistent with the retinal vasculopathy seen in patients infected with DENV; decreases in epithelial junctional protein expression, paralleling loss of integrity of the epithelium, provide a molecular basis for DENV-associated macular edema. These molecular processes present potential therapeutic targets for vision-threatening dengue retinopathy. © 2017 Jillian M. Carr et al. | |
dc.publisher | Hindawi Limited | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Unpaywall 20201031 | |
dc.subject | actin | |
dc.subject | antivirus agent | |
dc.subject | beta catenin | |
dc.subject | catenin beta 1 | |
dc.subject | interferon | |
dc.subject | protein ZO1 | |
dc.subject | unclassified drug | |
dc.subject | vascular adhesion protein 1 | |
dc.subject | Article | |
dc.subject | cell function | |
dc.subject | cytopathogenic effect | |
dc.subject | dengue | |
dc.subject | Dengue virus | |
dc.subject | Dengue virus 2 | |
dc.subject | gene overexpression | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | macular edema | |
dc.subject | protein expression | |
dc.subject | retina cell | |
dc.subject | retinopathy | |
dc.subject | virus replication | |
dc.subject | cell culture | |
dc.subject | cell line | |
dc.subject | cytology | |
dc.subject | Dengue virus | |
dc.subject | endothelium cell | |
dc.subject | epithelium cell | |
dc.subject | immunohistochemistry | |
dc.subject | immunology | |
dc.subject | metabolism | |
dc.subject | pathogenicity | |
dc.subject | physiology | |
dc.subject | retina | |
dc.subject | tight junction | |
dc.subject | virology | |
dc.subject | Cell Line | |
dc.subject | Cells, Cultured | |
dc.subject | Dengue Virus | |
dc.subject | Endothelial Cells | |
dc.subject | Epithelial Cells | |
dc.subject | Humans | |
dc.subject | Immunohistochemistry | |
dc.subject | Retina | |
dc.subject | Tight Junctions | |
dc.subject | Virus Replication | |
dc.type | Article | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.1155/2017/3164375 | |
dc.description.sourcetitle | Mediators of Inflammation | |
dc.description.volume | 2017 | |
dc.description.page | 3164375 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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