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Title: Derivation of Porcine Embryonic Stem-Like Cells from in Vitro-Produced Blastocyst-Stage Embryos
Authors: Hou, D.-R
Jin, Y
Nie, X.-W 
Zhang, M.-L
Ta, N
Zhao, L.-H
Yang, N
Chen, Y
Wu, Z.-Q
Jiang, H.-B
Li, Y.-R
Sun, Q.-Y
Dai, Y.-F
Li, R.-F
Keywords: biological marker
cell differentiation
cell shape
CFU counting
cluster analysis
embryoid body
embryonic stem cell
gene expression profiling
gene expression regulation
genetic transcription
in vitro fertilization
pluripotent stem cell
Cell Differentiation
Cell Shape
Cluster Analysis
Colony-Forming Units Assay
Embryoid Bodies
Embryonic Stem Cells
Fertilization in Vitro
Gene Expression Profiling
Gene Expression Regulation
Pluripotent Stem Cells
Sus scrofa
Transcription, Genetic
Issue Date: 2016
Citation: Hou, D.-R, Jin, Y, Nie, X.-W, Zhang, M.-L, Ta, N, Zhao, L.-H, Yang, N, Chen, Y, Wu, Z.-Q, Jiang, H.-B, Li, Y.-R, Sun, Q.-Y, Dai, Y.-F, Li, R.-F (2016). Derivation of Porcine Embryonic Stem-Like Cells from in Vitro-Produced Blastocyst-Stage Embryos. Scientific Reports 6 : 25838. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Efficient isolation of embryonic stem (ES) cells from pre-implantation porcine embryos has remained a challenge. Here, we describe the derivation of porcine embryonic stem-like cells (pESLCs) by seeding the isolated inner cell mass (ICM) from in vitro-produced porcine blastocyst into ?-MEM with basic fibroblast growth factor (bFGF). The pESL cells kept the normal karyotype and displayed flatten clones, similar in phenotype to human embryonic stem cells (hES cells) and rodent epiblast stem cells. These cells exhibited alkaline phosphatase (AP) activity and expressed pluripotency markers such as OCT4, NANOG, SOX2, SSEA-4, TRA-1-60, and TRA-1-81 as determined by both immunofluorescence and RT-PCR. Additionally, these cells formed embryoid body (EB), teratomas and also differentiated into 3 germ layers in vitro and in vivo. Microarray analysis showed the expression of the pluripotency markers, PODXL, REX1, SOX2, KLF5 and NR6A1, was significantly higher compared with porcine embryonic fibroblasts (PEF), but expression of OCT4, TBX3, REX1, LIN28A and DPPA5, was lower compared to the whole blastocysts or ICM of blastocyst. Our results showed that porcine embryonic stem-like cells can be established from in vitro-produced blastocyst-stage embryos, which promote porcine naive ES cells to be established.
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/srep25838
Rights: Attribution 4.0 International
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