Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.25903
Title: Plasmodium P36 determines host cell receptor usage during sporozoite invasion
Authors: Manzoni, G
Marinach, C
Topçu, S
Briquet, S
Grand, M
Tolle, M
Gransagne, M
Lescar, J 
Andolina, C
Franetich, J.-F
Zeisel, M.B
Huby, T
Rubinstein, E
Snounou, G
Mazier, D
Nosten, F
Baumert, T.F
Silvie, O
Keywords: CD81 antigen
cell surface protein
green fluorescent protein
peptides and proteins
protein p36
protein p52
scavenger receptor BI
small interfering RNA
unclassified drug
CD81 antigen
CD81 protein, human
membrane protein
protozoal protein
SCARB1 protein, human
scavenger receptor B
amino terminal sequence
animal model
Anopheles stephensi
Article
cell invasion
controlled study
female
flow cytometry
fluorescence activated cell sorting
fluorescence microscopy
gene deletion
genetic complementation
genetic transfection
genotype
host parasite interaction
human
human cell
immunofluorescence test
mouse
nonhuman
parasitosis
Plasmodium
Plasmodium berghei
Plasmodium falciparum
Plasmodium vivax
Plasmodium yoelii
polymerase chain reaction
sporozoite
structural bioinformatics
animal
cell line
endocytosis
growth, development and aging
host pathogen interaction
liver cell
metabolism
parasitology
rodent
sporozoite
Animals
Cell Line
Endocytosis
Hepatocytes
Host-Pathogen Interactions
Humans
Membrane Proteins
Plasmodium berghei
Plasmodium falciparum
Plasmodium vivax
Protozoan Proteins
Rodentia
Scavenger Receptors, Class B
Sporozoites
Tetraspanin 28
Issue Date: 2017
Citation: Manzoni, G, Marinach, C, Topçu, S, Briquet, S, Grand, M, Tolle, M, Gransagne, M, Lescar, J, Andolina, C, Franetich, J.-F, Zeisel, M.B, Huby, T, Rubinstein, E, Snounou, G, Mazier, D, Nosten, F, Baumert, T.F, Silvie, O (2017). Plasmodium P36 determines host cell receptor usage during sporozoite invasion. eLife 6 : e25903. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.25903
Rights: Attribution 4.0 International
Abstract: Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocytes remain poorly understood. Here we show that the two main species causing malaria in humans, Plasmodium falciparum and Plasmodium vivax, rely on two distinct host cell surface proteins, CD81 and the Scavenger Receptor BI (SR-BI), respectively, to infect hepatocytes. By contrast, CD81 and SR-BI fulfil redundant functions during infection by the rodent parasite P. berghei. Genetic analysis of sporozoite factors reveals the 6-cysteine domain protein P36 as a major parasite determinant of host cell receptor usage. Our data provide molecular insights into the invasion pathways used by different malaria parasites to infect hepatocytes, and establish a functional link between a sporozoite putative ligand and host cell receptors. © Manzoni et al.
Source Title: eLife
URI: https://scholarbank.nus.edu.sg/handle/10635/178677
ISSN: 2050084X
DOI: 10.7554/eLife.25903
Rights: Attribution 4.0 International
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