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https://doi.org/10.7554/eLife.24476
Title: | Functionally diverse human T cells recognize non-microbial antigens presented by MR1 | Authors: | Lepore, M Kalinichenko, A Calogero, S Kumar, P Paleja, B Schmaler, M Narang, V Zolezzi, F Poidinger, M Mori, L de Libero, G |
Keywords: | CD137 antigen CD69 antigen CD83 antigen CD86 antigen chemokine receptor CCR4 chemokine receptor CCR6 chemokine receptor CXCR3 early growth response factor 1 gamma interferon interferon regulatory factor 4 interleukin 10 interleukin 2 interleukin 3 interleukin 4 interleukin 5 interleukin 6 lymphotoxin major histocompatibility antigen class 1 major histocompatibility complex class 1 related gene protein mucin 2 protein c fos retinoid X receptor alpha T lymphocyte receptor alpha chain T lymphocyte receptor beta chain transcription factor FOXP3 transcription factor JunB transcription factor T bet transcriptome tumor necrosis factor unclassified drug unindexed drug antigen chemokine receptor cytokine HLA antigen class 1 minor histocompatibility antigen MR1 protein, human animal cell antigen recognition Article cell fractionation controlled study cytokine release down regulation enzyme linked immunosorbent assay flow cytometry fluorescence activated cell sorting gene expression gene transfer human human cell immunostimulation next generation sequencing nonhuman nucleotide sequence protein expression reverse transcription polymerase chain reaction RNA sequence T lymphocyte T lymphocyte activation upregulation Western blotting antigen presentation biosynthesis cell line immunology metabolism secretion (process) T lymphocyte Antigen Presentation Antigens Cell Line Cytokines Histocompatibility Antigens Class I Humans Minor Histocompatibility Antigens Receptors, Chemokine T-Lymphocytes |
Issue Date: | 2017 | Citation: | Lepore, M, Kalinichenko, A, Calogero, S, Kumar, P, Paleja, B, Schmaler, M, Narang, V, Zolezzi, F, Poidinger, M, Mori, L, de Libero, G (2017). Functionally diverse human T cells recognize non-microbial antigens presented by MR1. eLife 6 : e24476. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.24476 | Rights: | Attribution 4.0 International | Abstract: | MHC class I-related molecule MR1 presents riboflavin-and folate-related metabolites to mucosal-associated invariant T cells, but it is unknown whether MR1 can present alternative antigens to other T cell lineages. In healthy individuals we identified MR1-restricted T cells (named MR1T cells) displaying diverse TCRs and reacting to MR1-expressing cells in the absence of microbial ligands. Analysis of MR1T cell clones revealed specificity for distinct cell-derived antigens and alternative transcriptional strategies for metabolic programming, cell cycle control and functional polarization following antigen stimulation. Phenotypic and functional characterization of MR1T cell clones showed multiple chemokine receptor expression profiles and secretion of diverse effector molecules, suggesting functional heterogeneity. Accordingly, MR1T cells exhibited distinct T helper-like capacities upon MR1-dependent recognition of target cells expressing physiological levels of surface MR1. These data extend the role of MR1 beyond microbial antigen presentation and indicate MR1T cells are a normal part of the human T cell repertoire. © Lepore et al. | Source Title: | eLife | URI: | https://scholarbank.nus.edu.sg/handle/10635/178676 | ISSN: | 2050084X | DOI: | 10.7554/eLife.24476 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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