Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.24476
Title: Functionally diverse human T cells recognize non-microbial antigens presented by MR1
Authors: Lepore, M
Kalinichenko, A
Calogero, S
Kumar, P
Paleja, B
Schmaler, M
Narang, V
Zolezzi, F
Poidinger, M 
Mori, L
de Libero, G
Keywords: CD137 antigen
CD69 antigen
CD83 antigen
CD86 antigen
chemokine receptor CCR4
chemokine receptor CCR6
chemokine receptor CXCR3
early growth response factor 1
gamma interferon
interferon regulatory factor 4
interleukin 10
interleukin 2
interleukin 3
interleukin 4
interleukin 5
interleukin 6
lymphotoxin
major histocompatibility antigen class 1
major histocompatibility complex class 1 related gene protein
mucin 2
protein c fos
retinoid X receptor alpha
T lymphocyte receptor alpha chain
T lymphocyte receptor beta chain
transcription factor FOXP3
transcription factor JunB
transcription factor T bet
transcriptome
tumor necrosis factor
unclassified drug
unindexed drug
antigen
chemokine receptor
cytokine
HLA antigen class 1
minor histocompatibility antigen
MR1 protein, human
animal cell
antigen recognition
Article
cell fractionation
controlled study
cytokine release
down regulation
enzyme linked immunosorbent assay
flow cytometry
fluorescence activated cell sorting
gene expression
gene transfer
human
human cell
immunostimulation
next generation sequencing
nonhuman
nucleotide sequence
protein expression
reverse transcription polymerase chain reaction
RNA sequence
T lymphocyte
T lymphocyte activation
upregulation
Western blotting
antigen presentation
biosynthesis
cell line
immunology
metabolism
secretion (process)
T lymphocyte
Antigen Presentation
Antigens
Cell Line
Cytokines
Histocompatibility Antigens Class I
Humans
Minor Histocompatibility Antigens
Receptors, Chemokine
T-Lymphocytes
Issue Date: 2017
Citation: Lepore, M, Kalinichenko, A, Calogero, S, Kumar, P, Paleja, B, Schmaler, M, Narang, V, Zolezzi, F, Poidinger, M, Mori, L, de Libero, G (2017). Functionally diverse human T cells recognize non-microbial antigens presented by MR1. eLife 6 : e24476. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.24476
Rights: Attribution 4.0 International
Abstract: MHC class I-related molecule MR1 presents riboflavin-and folate-related metabolites to mucosal-associated invariant T cells, but it is unknown whether MR1 can present alternative antigens to other T cell lineages. In healthy individuals we identified MR1-restricted T cells (named MR1T cells) displaying diverse TCRs and reacting to MR1-expressing cells in the absence of microbial ligands. Analysis of MR1T cell clones revealed specificity for distinct cell-derived antigens and alternative transcriptional strategies for metabolic programming, cell cycle control and functional polarization following antigen stimulation. Phenotypic and functional characterization of MR1T cell clones showed multiple chemokine receptor expression profiles and secretion of diverse effector molecules, suggesting functional heterogeneity. Accordingly, MR1T cells exhibited distinct T helper-like capacities upon MR1-dependent recognition of target cells expressing physiological levels of surface MR1. These data extend the role of MR1 beyond microbial antigen presentation and indicate MR1T cells are a normal part of the human T cell repertoire. © Lepore et al.
Source Title: eLife
URI: https://scholarbank.nus.edu.sg/handle/10635/178676
ISSN: 2050084X
DOI: 10.7554/eLife.24476
Rights: Attribution 4.0 International
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