Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-11380-0
Title: Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
Authors: Chen, S
Liu, X 
Wang, N
Wang, X
Xiong, Q
Bo, E
Yu, X
Chen, S
Liu, L
Keywords: fibrillar collagen
animal
C57BL mouse
cornea
cornea cell
cornea endothelium
endothelium cell
feasibility study
female
innervation
minipig
optical coherence tomography
pig
procedures
Sprague Dawley rat
ultrastructure
Animals
Cornea
Corneal Keratocytes
Endothelial Cells
Endothelium, Corneal
Feasibility Studies
Female
Fibrillar Collagens
Mice, Inbred C57BL
Rats, Sprague-Dawley
Swine
Swine, Miniature
Tomography, Optical Coherence
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Chen, S, Liu, X, Wang, N, Wang, X, Xiong, Q, Bo, E, Yu, X, Chen, S, Liu, L (2017). Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography. Scientific Reports 7 (1) : 10752. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-11380-0
Rights: Attribution 4.0 International
Abstract: Diagnosis of corneal disease and challenges in corneal transplantation require comprehensive understanding of corneal anatomy, particularly that of the posterior cornea. Micro-optical coherence tomography (?OCT) is a potentially suitable tool to meet this need, owing to its ultrahigh isotropic spatial resolution, high image acquisition rate and depth priority scanning mode. In this study, we explored the ability of ?OCT to visualize micro-anatomical structures of the posterior cornea ex vivo and in vivo using small and large animals. ?OCT clearly delineated cornea layers and revealed micro-anatomical structures, including not only polygonal endothelial cells, stellate keratocytes, collagen fibres and corneal nerve fibres but also new structures such as the dome-shaped basolateral side of endothelial cells and lattice structures at the interface between endothelium and Descemet's membrane. Based on these observations, a short post-harvest longitudinal study was conducted on rat cornea to test the feasibility of using ?OCT to monitor the quality of endothelial cells. This study successfully reveals a series of morphological features and pathological changes in the posterior cornea at the cellular level in situ and in real time with ?OCT. These findings enrich knowledge of corneal anatomy and suggest that ?OCT may be a promising imaging tool in corneal transplantation. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178582
ISSN: 2045-2322
DOI: 10.1038/s41598-017-11380-0
Rights: Attribution 4.0 International
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