Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-14723-z
Title: Regulatory Compliant Tissue-Engineered Human Corneal Endothelial Grafts Restore Corneal Function of Rabbits with Bullous Keratopathy
Authors: Peh, G.S.L 
Ang, H.-P
Lwin, C.N
Adnan, K
George, B.L 
Seah, X.-Y
Lin, S.-J
Bhogal, M
Liu, Y.-C 
Tan, D.T 
Mehta, J.S 
Keywords: adolescent
adult
animal
cell culture technique
child
cornea disease
cornea endothelium
cornea stroma
cornea transplantation
cryopreservation
cytology
disease model
extracellular matrix
female
human
Leporidae
male
preschool child
procedures
tissue engineering
transplantation
Adolescent
Adult
Animals
Cell Culture Techniques
Child
Child, Preschool
Corneal Diseases
Corneal Stroma
Corneal Transplantation
Cryopreservation
Disease Models, Animal
Endothelium, Corneal
Extracellular Matrix
Female
Humans
Male
Rabbits
Tissue Engineering
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Peh, G.S.L, Ang, H.-P, Lwin, C.N, Adnan, K, George, B.L, Seah, X.-Y, Lin, S.-J, Bhogal, M, Liu, Y.-C, Tan, D.T, Mehta, J.S (2017). Regulatory Compliant Tissue-Engineered Human Corneal Endothelial Grafts Restore Corneal Function of Rabbits with Bullous Keratopathy. Scientific Reports 7 (1) : 14149. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-14723-z
Rights: Attribution 4.0 International
Abstract: Corneal transplantation is the only treatment available to restore vision for individuals with blindness due to corneal endothelial dysfunction. However, severe shortage of available donor corneas remains a global challenge. Functional regulatory compliant tissue-engineered corneal endothelial graft substitute can alleviate this reliance on cadaveric corneal graft material. Here, isolated primary human corneal endothelial cells (CEnCs) propagated using a dual media approach refined towards regulatory compliance showed expression of markers indicative of the human corneal endothelium, and can be tissue-engineered onto thin corneal stromal carriers. Both cellular function and clinical adaptability was demonstrated in a pre-clinical rabbit model of bullous keratopathy using a tissue-engineered endothelial keratoplasty (TE-EK) approach, adapted from routine endothelial keratoplasty procedure for corneal transplantation in human patients. Cornea thickness of rabbits receiving TE-EK graft gradually reduced over the first two weeks, and completely recovered to a thickness of approximately 400 ?m by the third week of transplantation, whereas corneas of control rabbits remained significantly thicker over 1,000 ?m (p < 0.05) throughout the course of the study. This study showed convincing evidence of the adaptability of the propagated CEnCs and their functionality via a TE-EK approach, which holds great promises in translating the use of cultured CEnCs into the clinic. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/178563
ISSN: 2045-2322
DOI: 10.1038/s41598-017-14723-z
Rights: Attribution 4.0 International
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