Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-018-21792-1
Title: | Conformational IgE Epitope Mapping of der p 2 and the Evaluations of Two Candidate Hypoallergens for Immunotherapy | Authors: | Reginald, K Chew, F.T |
Keywords: | allergen arthropod protein cytokine Dermatophagoides pteronyssinus antigen p 2 epitope house dust allergen immunoglobulin E immunoglobulin G vaccine animal Bagg albino mouse epitope mapping female human immunology immunotherapy mononuclear cell mouse procedures T lymphocyte Allergens Animals Antigens, Dermatophagoides Arthropod Proteins Cytokines Epitope Mapping Epitopes Female Humans Immunoglobulin E Immunoglobulin G Immunotherapy Leukocytes, Mononuclear Mice Mice, Inbred BALB C T-Lymphocytes Vaccines |
Issue Date: | 2018 | Publisher: | Nature Publishing Group | Citation: | Reginald, K, Chew, F.T (2018). Conformational IgE Epitope Mapping of der p 2 and the Evaluations of Two Candidate Hypoallergens for Immunotherapy. Scientific Reports 8 (1) : 3391. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-21792-1 | Rights: | Attribution 4.0 International | Abstract: | Epitope mapping of Der p 2, a clinically important dust-mite allergen is the first step in designing immunotherapy hypoallergen vaccine candidates. Twenty-one single alanine mutants of Der p 2 were generated and their secondary structure was analysed using circular dichroism spectra. Only one mutant, K96A resulted in a misfolded protein. All mutants were tested for serum IgE reactivity using serum from dust mite allergic individuals by immuno dot-blots. Mutations to five residues, N10, E25, K77, K96 and E102 consistently showed reduced IgE reactions compared to wild-Type Der p 2, and therefore these residues constitute the major IgE epitopes of Der p 2. Two mutants with consistent low IgE binding, K96A and E102A, were subsequently evaluated as hypoallergen candidates. IgG antibodies raised in mice against both mutants could inhibit human IgE-binding to WT Der p 2. Both mutants had intact T-cell epitopes as they were able to stimulate peripheral blood mononuclear cell proliferation similar to WT Der p 2. However, a switch in Th1:Th2 cytokine profile was not observed. In summary, we have identified the major conformational epitopes of Der p 2, and evaluated two Der p 2 hypoallergen vaccine candidates for immunotherapy. © 2018 The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/178428 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-018-21792-1 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1038_s41598-018-21792-1.pdf | 1.27 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License