Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-018-04461-9
Title: Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation
Authors: Ezzoukhry, Z
Henriet, E
Cordelières, F.P
Dupuy, J.-W
Maître, M
Gay, N
Di-Tommaso, S
Mercier, L
Goetz, J.G
Peter, M
Bard, F 
Moreau, V
Raymond, A.-A
Saltel, F
Keywords: biomarker
laser method
machinery
protein
proteomics
purification
article
contamination
laser capture microdissection
machine
mass spectrometry
podosome
protein function
proteomics
structure activity relation
validation process
animal
extracellular matrix
high performance liquid chromatography
human
laser capture microdissection
metabolism
mouse
neoplasm
NIH 3T3 cell line
pathology
podosome
procedures
protein synthesis
proteomics
tandem mass spectrometry
tumor cell line
actin
messenger RNA
tumor marker
Actins
Animals
Biomarkers, Tumor
Cell Line, Tumor
Chromatography, High Pressure Liquid
Extracellular Matrix
Humans
Laser Capture Microdissection
Mice
Neoplasms
NIH 3T3 Cells
Podosomes
Protein Biosynthesis
Proteomics
RNA, Messenger
Tandem Mass Spectrometry
Issue Date: 2018
Publisher: Nature Publishing Group
Citation: Ezzoukhry, Z, Henriet, E, Cordelières, F.P, Dupuy, J.-W, Maître, M, Gay, N, Di-Tommaso, S, Mercier, L, Goetz, J.G, Peter, M, Bard, F, Moreau, V, Raymond, A.-A, Saltel, F (2018). Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation. Nature Communications 9 (1) : 4461. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-04461-9
Rights: Attribution 4.0 International
Abstract: Invadosomes are F-actin-based structures involved in extracellular matrix degradation, cell invasion, and metastasis formation. Analyzing their proteome is crucial to decipher their molecular composition, to understand their mechanisms, and to find specific elements to target them. However, the specific analysis of invadosomes is challenging, because it is difficult to maintain their integrity during isolation. In addition, classical purification methods often suffer from contaminations, which may impair data validation. To ensure the specific identification of invadosome components, we here develop a method that combines laser microdissection and mass spectrometry, enabling the analysis of subcellular structures in their native state based on low amounts of input material. Using this combinatorial method, we show that invadosomes contain specific components of the translational machinery, in addition to known marker proteins. Moreover, functional validation reveals that protein translation activity is an inherent property of invadosomes, which is required to maintain invadosome structure and activity. © 2018 The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/178410
ISSN: 2041-1723
DOI: 10.1038/s41467-018-04461-9
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_s41467-018-04461-9.pdf3.39 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

12
checked on Nov 19, 2020

Page view(s)

23
checked on Nov 19, 2020

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons