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Title: Novel application of In Vivo Micro-Optical Coherence Tomography to assess Cornea scarring in an Animal Model
Authors: Ang, M
Devarajan, K
Das, S
Yam, G.H.F 
Htoon, H.M 
Chen, S
Liu, X 
Liu, L
Girard, M 
Mehta, J.S 
Keywords: animal
animal model
C57BL mouse
cornea injury
optical coherence tomography
pilot study
Corneal Injuries
Mice, Inbred C57BL
Models, Animal
Pilot Projects
Tomography, Optical Coherence
Issue Date: 2018
Publisher: Nature Publishing Group
Citation: Ang, M, Devarajan, K, Das, S, Yam, G.H.F, Htoon, H.M, Chen, S, Liu, X, Liu, L, Girard, M, Mehta, J.S (2018). Novel application of In Vivo Micro-Optical Coherence Tomography to assess Cornea scarring in an Animal Model. Scientific Reports 8 (1) : 11483. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: This pilot study uses a micro-optical coherence tomography (micro-OCT) system with ~1 ?m axial resolution specifically to image the cornea and corneal scars in vivo. We used an established murine corneal scar model by irregular phototherapeutic keratectomy in ten C57BL/6 mice, with serial imaging using the micro-OCT and compared to anterior segment (AS-OCT) (RTvue, Optovue, Fremont, CA) before and after scar induction. Main outcome was agreement between the AS-OCT and micro-OCT using Bland-Altman plots (95% limits of agreement, LoA).We analysed 10 control eyes and 10 eyes with corneal scars and found that there was good agreement between AS-OCT and micro-OCT (P > 0.05) LOA: lower limit ?14 µm (95% CI: ?19 to ?8.8 µm) upper limit 23 µm (95% CI: 18 to 28.5 µm) in terms of central corneal thickness. There was also good agreement between AS-OCT and micro-OCT in terms of corneal scar measurements (P > 0.5; correlation coefficient >0.99) LOA lower limit ?2.1 µm (95% CI: ?2.8 to ?1.5 µm); upper limit 1.8 µm (95% CI: 1.1 to 2.4 µm). Our pilot study suggests that this novel in vivo micro-OCT imaging technique was able to measure central corneal thickness and scar thickness in agreement with current AS-OCT techniques. © 2018, The Author(s).
Source Title: Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-018-29761-4
Rights: Attribution 4.0 International
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