Please use this identifier to cite or link to this item: https://doi.org/10.1038/msb.2011.84
Title: The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells
Authors: Munoz, J
Low, T.Y
Kok, Y.J
Chin, A
Frese, C.K
Ding, V
Choo, A 
Heck, A.J.R
Keywords: proteome
proteome
animal cell
animal experiment
animal model
article
cell adhesion
embryonic stem cell
female
fibroblast culture
mass spectrometry
metabolism
mouse
nonhuman
pluripotent stem cell
priority journal
protein expression
quantitative analysis
cell line
cytology
DNA microarray
embryonic stem cell
gene expression profiling
genetics
human
methodology
Cell Line
Embryonic Stem Cells
Gene Expression Profiling
Humans
Mass Spectrometry
Oligonucleotide Array Sequence Analysis
Pluripotent Stem Cells
Proteome
Issue Date: 2011
Publisher: EMBO Press
Citation: Munoz, J, Low, T.Y, Kok, Y.J, Chin, A, Frese, C.K, Ding, V, Choo, A, Heck, A.J.R (2011). The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. Molecular Systems Biology 7 : 550. ScholarBank@NUS Repository. https://doi.org/10.1038/msb.2011.84
Rights: Attribution 4.0 International
Abstract: Assessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in-depth quantitative mass spectrometry-based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of hESCs and hiPSCS at the proteome level as 97.8% of the proteins were found unchanged. Nevertheless, a small group of 58 proteins, mainly related to metabolism, antigen processing and cell adhesion, was found significantly differentially expressed between hiPSCs and hESCs. A comparison of the regulated proteins with previously published transcriptomic studies showed a low overlap, highlighting the emerging notion that differences between both pluripotent cell lines rather reflect experimental conditions than a recurrent molecular signature. © 2011 EMBO and Macmillan Publishers Limited.
Source Title: Molecular Systems Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/178165
ISSN: 1744-4292
DOI: 10.1038/msb.2011.84
Rights: Attribution 4.0 International
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