Please use this identifier to cite or link to this item: https://doi.org/10.1186/1749-8546-7-16
Title: Serum proteomic, peptidomic and metabolomic profiles in myasthenia gravis patients during treatment with Qiangji Jianli Fang
Authors: Wang, C
Lu, Y 
Chen, Z
Liu, X
Lin, H
Zhao, H
Chen, J
Kwan, Y
Ngai, S
Keywords: 4 aminobutyric acid
albumin
alpha 2 macroglobulin
biliverdin
blood clotting factor 13a
Chinese drug
cholinergic receptor stimulating agent
complement component C3
complement component C4b
gelsolin
haptoglobin
hemopexin
kallidin
phytosphingosine
prothrombin
qiangji jianli fang
thromboxane B2
unclassified drug
adult
albumin blood level
article
clinical article
controlled study
drug effect
female
Fourier transform mass spectrometry
high throughput screening
human
liquid chromatography
male
matrix assisted laser desorption ionization time of flight mass spectrometry
metabolomics
myasthenia gravis
peptidomics
proteomics
two dimensional gel electrophoresis
Issue Date: 2012
Publisher: BMC
Citation: Wang, C, Lu, Y, Chen, Z, Liu, X, Lin, H, Zhao, H, Chen, J, Kwan, Y, Ngai, S (2012). Serum proteomic, peptidomic and metabolomic profiles in myasthenia gravis patients during treatment with Qiangji Jianli Fang. Chinese Medicine (United Kingdom) 7 : 16. ScholarBank@NUS Repository. https://doi.org/10.1186/1749-8546-7-16
Rights: Attribution 4.0 International
Abstract: Background: Qiangji Jianli Fang (QJF) has been used for treatment of myasthenia gravis (MG) in China. However, our understanding of the effects of QJF against MG at the molecular level is limited. This study aims to investigate the effects of QJF treatment of MG patients on the protein, peptide and metabolite levels in serum.Methods: High-throughput proteomic, peptidomic and metabolomic techniques were applied to investigate serum samples from 21 healthy individuals and 47 MG patients before and after QJF treatment via two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time of flight mass spectrometry and liquid chromatography Fourier transform mass spectrometry, respectively.Results: After QJF treatment, the expression levels of peptides m/z 1865.019, 2021.128 and 1211.668 of complement C3f increased (P = 0.004, P = 0.001 and P = 0.043, respectively), while that of peptide m/z 1739.931 of component C4b decreased (P = 0.043), in the serum of MG patients. The levels of ?-aminobutyric acid (P = 0.000) and coenzyme Q4 (P = 0.000) resumed their normal states.Conclusion: QJF could inhibit the activity of the complement system and restore the normal levels of metabolites. © 2012 Wang et al.; licensee BioMed Central Ltd.
Source Title: Chinese Medicine (United Kingdom)
URI: https://scholarbank.nus.edu.sg/handle/10635/178143
ISSN: 1749-8546
DOI: 10.1186/1749-8546-7-16
Rights: Attribution 4.0 International
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