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https://doi.org/10.3390/toxins10090373
Title: | Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps | Authors: | Chapeaurouge, A Silva, A Carvalho, P McCleary, R.J.R Modahl, C.M Perales, J Kini, R.M Mackessy, S.P |
Keywords: | proteome reptilian protein snake venom animal Bungarus chemistry female male proteomics tandem mass spectrometry Animals Bungarus Elapid Venoms Female Male Proteome Proteomics Reptilian Proteins Tandem Mass Spectrometry |
Issue Date: | 2018 | Citation: | Chapeaurouge, A, Silva, A, Carvalho, P, McCleary, R.J.R, Modahl, C.M, Perales, J, Kini, R.M, Mackessy, S.P (2018). Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps. Toxins 10 (9). ScholarBank@NUS Repository. https://doi.org/10.3390/toxins10090373 | Rights: | Attribution 4.0 International | Abstract: | The use of -omics technologies allows for the characterization of snake venom composition at a fast rate and at high levels of detail. In the present study, we investigated the protein content of Red-headed Krait (Bungarus flaviceps) venom. This analysis revealed a high diversity of snake venom protein families, as evidenced by high-throughput mass spectrometric analysis. We found all six venom protein families previously reported in a transcriptome study of the venom gland of B. flaviceps, including phospholipases A? (PLA?s), Kunitz-type serine proteinase inhibitors (KSPIs), three-finger toxins (3FTxs), cysteine-rich secretory proteins (CRISPs), snaclecs, and natriuretic peptides. A combined approach of automated database searches and de novo sequencing of tandem mass spectra, followed by sequence similarity searches, revealed the presence of 12 additional toxin families. De novo sequencing alone was able to identify 58 additional peptides, and this approach contributed significantly to the comprehensive description of the venom. Abundant protein families comprise 3FTxs (22.3%), KSPIs (19%), acetylcholinesterases (12.6%), PLA?s (11.9%), venom endothelial growth factors (VEGFs, 8.4%), nucleotidases (4.3%), and C-type lectin-like proteins (snaclecs, 3.3%); an additional 11 toxin families are present at significantly lower concentrations, including complement depleting factors, a family not previously detected in Bungarus venoms. The utility of a multifaceted approach toward unraveling the proteome of snake venoms, employed here, allowed detection of even minor venom components. This more in-depth knowledge of the composition of B. flaviceps venom facilitates a better understanding of snake venom molecular evolution, in turn contributing to more effective treatment of krait bites. | Source Title: | Toxins | URI: | https://scholarbank.nus.edu.sg/handle/10635/177838 | ISSN: | 20726651 | DOI: | 10.3390/toxins10090373 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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