Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms4195
Title: Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment
Authors: Rodin, S
Antonsson, L
Niaudet, C
Simonson, O.E
Salmela, E
Hansson, E.M
Domogatskaya, A
Xiao, Z
Damdimopoulou, P
Sheikhi, M
Inzunza, J
Nilsson, A.-S
Baker, D
Kuiper, R
Sun, Y
Blennow, E
Nordenskjöld, M
Grinnemo, K.-H
Kere, J
Betsholtz, C
Hovatta, O
Tryggvason, K 
Keywords: laminin
laminin 521
unclassified drug
uvomorulin
cadherin
laminin
laminin 11 protein, human
phosphatidylinositol 3 kinase
protein kinase B
very late activation antigen 6
biotechnology
embryo
inhibitor
phenotype
protein
recombination
article
blastoma
cell cloning
cell renewal
cell survival
controlled study
embryo
embryonic stem cell
human
human cell
inner cell mass
stem cell niche
xeno-free culture
culture technique
embryonic stem cell
karyotyping
metabolism
physiology
Cadherins
Cell Culture Techniques
Embryonic Stem Cells
Humans
Integrin alpha6beta1
Karyotyping
Laminin
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
Issue Date: 2014
Citation: Rodin, S, Antonsson, L, Niaudet, C, Simonson, O.E, Salmela, E, Hansson, E.M, Domogatskaya, A, Xiao, Z, Damdimopoulou, P, Sheikhi, M, Inzunza, J, Nilsson, A.-S, Baker, D, Kuiper, R, Sun, Y, Blennow, E, Nordenskjöld, M, Grinnemo, K.-H, Kere, J, Betsholtz, C, Hovatta, O, Tryggvason, K (2014). Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nature Communications 5 : 3195. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms4195
Rights: Attribution 4.0 International
Abstract: Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes. © 2014 Macmillan Publishers Limited. All rights reserved.
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/177772
ISSN: 20411723
DOI: 10.1038/ncomms4195
Rights: Attribution 4.0 International
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