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https://doi.org/10.1038/ncomms4195
Title: | Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment | Authors: | Rodin, S Antonsson, L Niaudet, C Simonson, O.E Salmela, E Hansson, E.M Domogatskaya, A Xiao, Z Damdimopoulou, P Sheikhi, M Inzunza, J Nilsson, A.-S Baker, D Kuiper, R Sun, Y Blennow, E Nordenskjöld, M Grinnemo, K.-H Kere, J Betsholtz, C Hovatta, O Tryggvason, K |
Keywords: | laminin laminin 521 unclassified drug uvomorulin cadherin laminin laminin 11 protein, human phosphatidylinositol 3 kinase protein kinase B very late activation antigen 6 biotechnology embryo inhibitor phenotype protein recombination article blastoma cell cloning cell renewal cell survival controlled study embryo embryonic stem cell human human cell inner cell mass stem cell niche xeno-free culture culture technique embryonic stem cell karyotyping metabolism physiology Cadherins Cell Culture Techniques Embryonic Stem Cells Humans Integrin alpha6beta1 Karyotyping Laminin Phosphatidylinositol 3-Kinases Proto-Oncogene Proteins c-akt |
Issue Date: | 2014 | Citation: | Rodin, S, Antonsson, L, Niaudet, C, Simonson, O.E, Salmela, E, Hansson, E.M, Domogatskaya, A, Xiao, Z, Damdimopoulou, P, Sheikhi, M, Inzunza, J, Nilsson, A.-S, Baker, D, Kuiper, R, Sun, Y, Blennow, E, Nordenskjöld, M, Grinnemo, K.-H, Kere, J, Betsholtz, C, Hovatta, O, Tryggvason, K (2014). Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nature Communications 5 : 3195. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms4195 | Rights: | Attribution 4.0 International | Abstract: | Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes. © 2014 Macmillan Publishers Limited. All rights reserved. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/177772 | ISSN: | 20411723 | DOI: | 10.1038/ncomms4195 | Rights: | Attribution 4.0 International |
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