Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms15011418
Title: Circulating MicroRNAs as biomarkers of acute stroke
Authors: Sepramaniam, S 
Tan, J.-R 
Tan, K.-S
De Silva, D.A
Tavintharan, S
Woon, F.-P
Wang, C.-W
Yong, F.-L
Karolina, D.-S 
Kaur, P 
Liu, F.-J 
Lim, K.-Y 
Armugam, A 
Jeyaseelan, K 
Keywords: microRNA
microRNA 125 2
microRNA 125b 2
microRNA 145
microRNA 210
microRNA 27a
microRNA 422
microRNA 422a
microRNA 627
microRNA 920
unclassified drug
adult
artery
article
brain ischemia
cardioembolic stroke
cerebrovascular accident
cluster analysis
controlled study
diagnostic accuracy
gene expression
human
major clinical study
male
metabolic syndrome X
microarray analysis
middle aged
middle cerebral artery occlusion
nonhuman
principal component analysis
rat
real time polymerase chain reaction
receiver operating characteristic
RNA isolation
Adult
Animals
Biological Markers
Brain Ischemia
Case-Control Studies
Female
Humans
Male
MicroRNAs
Middle Aged
Rats
Stroke
Issue Date: 2014
Citation: Sepramaniam, S, Tan, J.-R, Tan, K.-S, De Silva, D.A, Tavintharan, S, Woon, F.-P, Wang, C.-W, Yong, F.-L, Karolina, D.-S, Kaur, P, Liu, F.-J, Lim, K.-Y, Armugam, A, Jeyaseelan, K (2014). Circulating MicroRNAs as biomarkers of acute stroke. International Journal of Molecular Sciences 15 (1) : 1418-1432. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms15011418
Abstract: MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pathology, identifying stroke specific microRNAs has been a challenge. This study shows that microRNA profiles reflect not only the temporal progression of stroke but also the specific etiologies. A panel of 32 microRNAs, which could differentiate stroke etiologies during acute phase was identified and verified using a customized TaqMan Low Density Array (TLDA). Furthermore we also found 5 microRNAs, miR-125b-2*, -27a*, -422a, -488 and -627 to be consistently altered in acute stroke irrespective of age or severity or confounding metabolic complications. Differential expression of these 5 microRNAs was also observed in rat stroke models. Hence, their specificity to the stroke pathology emphasizes the possibility of developing these microRNAs into accurate and useful tools for diagnosis of stroke. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
Source Title: International Journal of Molecular Sciences
URI: https://scholarbank.nus.edu.sg/handle/10635/176169
ISSN: 1661-6596
DOI: 10.3390/ijms15011418
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