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https://doi.org/10.3390/ijms15011418
Title: | Circulating MicroRNAs as biomarkers of acute stroke |
Authors: | Sepramaniam, S Tan, J.-R Tan, K.-S De Silva, D.A Tavintharan, S Woon, F.-P Wang, C.-W Yong, F.-L Karolina, D.-S Kaur, P Liu, F.-J Lim, K.-Y Armugam, A Jeyaseelan, K |
Keywords: | microRNA microRNA 125 2 microRNA 125b 2 microRNA 145 microRNA 210 microRNA 27a microRNA 422 microRNA 422a microRNA 627 microRNA 920 unclassified drug adult artery article brain ischemia cardioembolic stroke cerebrovascular accident cluster analysis controlled study diagnostic accuracy gene expression human major clinical study male metabolic syndrome X microarray analysis middle aged middle cerebral artery occlusion nonhuman principal component analysis rat real time polymerase chain reaction receiver operating characteristic RNA isolation Adult Animals Biological Markers Brain Ischemia Case-Control Studies Female Humans Male MicroRNAs Middle Aged Rats Stroke |
Issue Date: | 2014 |
Citation: | Sepramaniam, S, Tan, J.-R, Tan, K.-S, De Silva, D.A, Tavintharan, S, Woon, F.-P, Wang, C.-W, Yong, F.-L, Karolina, D.-S, Kaur, P, Liu, F.-J, Lim, K.-Y, Armugam, A, Jeyaseelan, K (2014). Circulating MicroRNAs as biomarkers of acute stroke. International Journal of Molecular Sciences 15 (1) : 1418-1432. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms15011418 |
Abstract: | MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pathology, identifying stroke specific microRNAs has been a challenge. This study shows that microRNA profiles reflect not only the temporal progression of stroke but also the specific etiologies. A panel of 32 microRNAs, which could differentiate stroke etiologies during acute phase was identified and verified using a customized TaqMan Low Density Array (TLDA). Furthermore we also found 5 microRNAs, miR-125b-2*, -27a*, -422a, -488 and -627 to be consistently altered in acute stroke irrespective of age or severity or confounding metabolic complications. Differential expression of these 5 microRNAs was also observed in rat stroke models. Hence, their specificity to the stroke pathology emphasizes the possibility of developing these microRNAs into accurate and useful tools for diagnosis of stroke. © 2014 by the authors; licensee MDPI, Basel, Switzerland. |
Source Title: | International Journal of Molecular Sciences |
URI: | https://scholarbank.nus.edu.sg/handle/10635/176169 |
ISSN: | 1661-6596 |
DOI: | 10.3390/ijms15011418 |
Appears in Collections: | Elements Staff Publications |
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