Please use this identifier to cite or link to this item: https://doi.org/10.1128/mSystems.00136-17
Title: Metagenomics reveals the influence of land use and rain on the benthic microbial communities in a tropical urban waterway
Authors: Saxena, G 
Mitra, S
Marzinelli, E.M
Xie, C
Wei, T.J
Steinberg, P.D
Williams, R.B.H 
Kjelleberg, S
Lauro, F.M
Swarup, S 
Keywords: rain
Actinobacteria
Article
Bacteroidetes
Beggiatoa
benthos
carbohydrate metabolism
carbon fixation
carbon metabolism
catchment
Coleofasciculus
community structure
cyanobacterium
gene function
industrial area
land use
metagenomics
microbial community
nitrogen cycling
nitrogen fixation
Nitrosopumilus
Nitrosospira
Nitrosovibrio
Nitrospira
nonhuman
nucleic acid metabolism
population abundance
protein metabolism
Proteobacteria
Singapore
species composition
Sulfuritalea
urban area
water supply
Issue Date: 2018
Citation: Saxena, G, Mitra, S, Marzinelli, E.M, Xie, C, Wei, T.J, Steinberg, P.D, Williams, R.B.H, Kjelleberg, S, Lauro, F.M, Swarup, S (2018). Metagenomics reveals the influence of land use and rain on the benthic microbial communities in a tropical urban waterway. mSystems 3 (3) : e0013617. ScholarBank@NUS Repository. https://doi.org/10.1128/mSystems.00136-17
Abstract: Growing demands for potable water have led to extensive reliance on waterways in tropical megacities. Attempts to manage these waterways in an environmentally sustainable way generally lack an understanding of microbial processes and how they are influenced by urban factors, such as land use and rain. Here, we describe the composition and functional potential of benthic microbial communities from an urban waterway network and analyze the effects of land use and rain perturbations on these communities. With a sequence depth of 3 billion reads from 48 samples, these metagenomes represent nearly full coverage of microbial communities. The predominant taxa in these waterways were Nitrospira and Coleofasciculus, indicating the presence of nitrogen and carbon fixation in this system. Gene functions from carbohydrate, protein, and nucleic acid metabolism suggest the presence of primary and secondary productivity in such nutrient-deficient systems. Comparison of microbial communities by land use type and rain showed that while there are significant differences in microbial communities in land use, differences due to rain perturbations were rain event specific. The more diverse microbial communities in the residential areas featured a higher abundance of reads assigned to genes related to community competition. However, the less diverse communities from industrial areas showed a higher abundance of reads assigned to specialized functions such as organic remediation. Finally, our study demonstrates that microbially diverse populations in well-managed waterways, where contaminant levels are within defined limits, are comparable to those in other relatively undisturbed freshwater systems. IMPORTANCE Unravelling the microbial metagenomes of urban waterway sediments suggest that well-managed urban waterways have the potential to support diverse sedimentary microbial communities, similar to those of undisturbed natural freshwaters. Despite the fact that these urban waterways are well managed, our study shows that environmental pressures from land use and rain perturbations play a role in shaping the structure and functions of microbial communities in these waterways. We propose that although pulsed disturbances, such as rain perturbations, influence microbial communities, press disturbances, including land usage history, have a long-term and stronger influence on microbial communities. Our study found that the functions of microbial communities were less affected by environmental factors than the structure of microbial communities was, indicating that core microbial functions largely remain conserved in challenging environments. Copyright © 2018 Saxena et al.
Source Title: mSystems
URI: https://scholarbank.nus.edu.sg/handle/10635/176046
ISSN: 2379-5077
DOI: 10.1128/mSystems.00136-17
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