Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep10521
Title: Characterization of a xylanase-producing Cellvibrio mixtus strain J3-8 and its genome analysis
Authors: Wu, Y.-R 
He, J 
Keywords: bacterial protein
recombinant protein
RNA 16S
xylan 1,4 beta xylosidase
amino acid sequence
animal
bacterial genome
biosynthesis
Cellvibrio
chemistry
classification
enzymology
Escherichia coli
genetics
isolation and purification
metabolism
microbiology
molecular cloning
molecular genetics
phylogeny
sequence alignment
sequence homology
snail
Amino Acid Sequence
Animals
Bacterial Proteins
Cellvibrio
Cloning, Molecular
Escherichia coli
Genome, Bacterial
Molecular Sequence Data
Phylogeny
Recombinant Proteins
RNA, Ribosomal, 16S
Sequence Alignment
Sequence Homology, Amino Acid
Snails
Xylosidases
Issue Date: 2015
Citation: Wu, Y.-R, He, J (2015). Characterization of a xylanase-producing Cellvibrio mixtus strain J3-8 and its genome analysis. Scientific Reports 5 : 10521. ScholarBank@NUS Repository. https://doi.org/10.1038/srep10521
Abstract: Cellvibrio mixtus strain J3-8 is a gram-negative, xylanase-producing aerobic soil bacterium isolated from giant snails in Singapore. It is able to produce up to 10.1 U ml-1 of xylanase, which is comparable to xylanase production from known bacterial and fungal strains. Genome sequence analysis of strain J3-8 reveals that the assembled draft genome contains 5,171,890 bp with a G + C content of 46.66%, while open reading frame (ORF) annotations indicate a high density of genes encoding glycoside hydrolase (GH) families involved in (hemi)cellulose hydrolysis. On the basis of 15 identified putative xylanolytic genes, one metabolic pathway in strain J3-8 is constructed for utilization of xylan. In addition, a 1,083 bp xylanase gene from strain J3-8 represents a new member of GH11 family. This gene is verified to be novel via phylogenetic analysis. To utilize this novel gene for hydrolysis of xylan to xylose, it is expressed in recombinant E. coli and characterized for its hydrolytic activity. This study shows that strain J3-8 is a potential candidate for hydrolysis of lignocellulosic materials.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175996
ISSN: 2045-2322
DOI: 10.1038/srep10521
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