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https://doi.org/10.1038/srep09737
| Title: | Contrasting expression patterns of coding and noncoding parts of the human genome upon oxidative stress | Authors: | Giannakakis, A Zhang, J Jenjaroenpun, P Nama, S Zainolabidin, N Aau, M.Y Yarmishyn, A.A Vaz, C Ivshina, A.V Grinchuk, O.V Voorhoeve, M Vardy, L.A Sampath, P Kuznetsov, V.A Kurochkin, I.V Guccione, E |
Keywords: | complementary RNA DNA directed RNA polymerase III long untranslated RNA messenger RNA RNA polymerase II transcription factor transcriptome untranslated RNA binding site biology cell line chromatin immunoprecipitation classification fibroblast gene expression profiling gene expression regulation genetics high throughput sequencing human human genome metabolism oxidative stress procedures promoter region protein synthesis Binding Sites Cell Line Chromatin Immunoprecipitation Computational Biology Fibroblasts Gene Expression Profiling Gene Expression Regulation Genome, Human High-Throughput Nucleotide Sequencing Humans Oxidative Stress Promoter Regions, Genetic Protein Biosynthesis RNA Polymerase II RNA Polymerase III RNA, Antisense RNA, Long Noncoding RNA, Messenger RNA, Untranslated Transcription Factors Transcriptome |
Issue Date: | 2015 | Citation: | Giannakakis, A, Zhang, J, Jenjaroenpun, P, Nama, S, Zainolabidin, N, Aau, M.Y, Yarmishyn, A.A, Vaz, C, Ivshina, A.V, Grinchuk, O.V, Voorhoeve, M, Vardy, L.A, Sampath, P, Kuznetsov, V.A, Kurochkin, I.V, Guccione, E (2015). Contrasting expression patterns of coding and noncoding parts of the human genome upon oxidative stress. Scientific Reports 5 : 9737. ScholarBank@NUS Repository. https://doi.org/10.1038/srep09737 | Abstract: | Oxidative stress (OS) is caused by an imbalance between pro- and anti-oxidant reactions leading to accumulation of reactive oxygen species within cells. We here investigate the effect of OS on the transcriptome of human fibroblasts. OS causes a rapid and transient global induction of transcription characterized by pausing of RNA polymerase II (PolII) in both directions, at specific promoters, within 30 minutes of the OS response. In contrast to protein-coding genes, which are commonly down-regulated, this novel divergent, PolII pausing-phenomenon leads to the generation of thousands of long noncoding RNAs (lncRNAs) with promoter-associated antisense lncRNAs transcripts (si-paancRNAs) representing the major group of stress-induced transcripts. OS causes transient dynamics of si-lncRNAs in nucleus and cytosol, leading to their accumulation at polysomes, in contrast to mRNAs, which get depleted from polysomes. We propose that si-lncRNAs represent a novel component of the transcriptional stress that is known to determine the outcome of immediate-early and later cellular stress responses and we provide insights on the fate of those novel mature lncRNA transcripts by showing that their association with polysomal complexes is significantly increased in OS. | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/175994 | ISSN: | 2045-2322 | DOI: | 10.1038/srep09737 |
| Appears in Collections: | Elements Staff Publications |
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