Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep06701
Title: A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum
Authors: Arai, S 
Lee, S.-C
Zhai, D 
Suzuki, M
Chang, Y.T 
Keywords: fluorescent dye
nanoparticle
chemistry
cytoplasm
endoplasmic reticulum
HeLa cell line
human
metabolism
temperature
thermometer
Cytoplasm
Endoplasmic Reticulum
Fluorescent Dyes
HeLa Cells
Humans
Nanoparticles
Temperature
Thermometers
Issue Date: 2014
Publisher: Nature Publishing Group
Citation: Arai, S, Lee, S.-C, Zhai, D, Suzuki, M, Chang, Y.T (2014). A molecular fluorescent probe for targeted visualization of temperature at the endoplasmic reticulum. Scientific Reports 4 : 6701. ScholarBank@NUS Repository. https://doi.org/10.1038/srep06701
Abstract: The dynamics of cellular heat production and propagation remains elusive at a subcellular level. Here we report the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow), with the highest sensitivity reported so far (3.9%/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca2+ changes in HeLa cells. Our thermometer anchored at nearly-zero distance from the ER, i.e. the heat source, allowed the detection of the heat as it readily dissipated, and revealed the dynamics of heat production in real time at a subcellular level.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175537
ISSN: 20452322
DOI: 10.1038/srep06701
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