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https://doi.org/10.18632/oncotarget.3622
Title: | Nimotuzumab increases the anti-tumor effect of photodynamic therapy in an oral tumor model | Authors: | Bhuvaneswari, R Ng, Q.F Thong, P.S.P Soo, K.-C |
Keywords: | CD31 antigen cetuximab chlorin e6 epidermal growth factor receptor Ki 67 antigen nimotuzumab photosensitizing agent unclassified drug EGFR protein, human epidermal growth factor receptor monoclonal antibody nimotuzumab phytochlorin porphyrin radiosensitizing agent animal experiment animal model antiangiogenic activity antineoplastic activity antiproliferative activity apoptosis Article cancer inhibition cancer size cell invasion chemotaxis concentration response controlled study down regulation drug potentiation drug safety endothelium cell extracellular matrix HSC 3 cell line human human cell immunohistochemistry kidney function light exposure liver function migration inhibition mouth squamous cell carcinoma nonhuman oral cancer cell line photodynamic therapy SCC 25 cell line therapy effect treatment response tumor xenograft umbilical vein endothelial cell animal antagonists and inhibitors Bagg albino mouse biosynthesis Carcinoma, Squamous Cell cell proliferation drug effects drug screening enzymology Head and Neck Neoplasms mouse Mouth Neoplasms multimodality cancer therapy nude mouse pathology photochemotherapy procedures randomization tumor cell line Animals Antibodies, Monoclonal, Humanized Carcinoma, Squamous Cell Cell Line, Tumor Cell Proliferation Combined Modality Therapy Drug Synergism Head and Neck Neoplasms Human Umbilical Vein Endothelial Cells Humans Mice Mice, Inbred BALB C Mice, Nude Mouth Neoplasms Photochemotherapy Porphyrins Radiation-Sensitizing Agents Random Allocation Receptor, Epidermal Growth Factor Xenograft Model Antitumor Assays |
Issue Date: | 2015 | Publisher: | Impact Journals LLC | Citation: | Bhuvaneswari, R, Ng, Q.F, Thong, P.S.P, Soo, K.-C (2015). Nimotuzumab increases the anti-tumor effect of photodynamic therapy in an oral tumor model. Oncotarget 6 (15) : 13487-13505. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.3622 | Abstract: | Oral squamous cell carcinoma (OSCC) represents 90% of all oral cancers and is characterized with poor prognosis and low survival rate. Epidermal growth factor receptor (EGFR) is highly expressed in oral cancer and is a target for cancer therapy and prevention. In this present work, we evaluate the efficacy of photodynamic therapy (PDT) in combination with an EGFR inhibitor, nimotuzumab in oral cancer cell lines and OSCC xenograft tumor model. PDT is a promising and minimally invasive treatment modality that involves the interaction of a photosensitizer, molecular oxygen and light to destroy tumors. We demonstrated that EGFR inhibitors nimotuzumab and cetuximab exhibits anti-angiogenic properties by inhibiting the migration and invasion of oral cancer cell lines and human endothelial cells. The EGFR inhibitors also significantly reduced tube formation of endothelial cells. Chlorin e6-PDT in combination with nimotuzumab and cetuximab reduced cell proliferation in different oral cancer and endothelial cells. Furthermore, our in vivo studies showed that the combination therapy of PDT and nimotuzumab synergistically delayed tumor growth when compared with control and PDT treated tumors. Downregulation of EGFR, Ki-67 and CD31 was observed in the tumors treated with combination therapy. Analysis of the liver and kidney function markers showed no treatment related toxicity. In conclusion, PDT outcome of oral cancer can be improved when combined with EGFR inhibitor nimotuzumab. | Source Title: | Oncotarget | URI: | https://scholarbank.nus.edu.sg/handle/10635/175527 | ISSN: | 19492553 | DOI: | 10.18632/oncotarget.3622 |
Appears in Collections: | Staff Publications Elements |
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