Please use this identifier to cite or link to this item:
https://doi.org/10.1038/ncomms7111
Title: | Mechanics of epithelial closure over non-adherent environments | Authors: | Vedula, S.R.K Peyret, G Cheddadi, I Chen, T Brugués, A Hirata, H Lopez-Menendez, H Toyama, Y Neves De Almeida, L Trepat, X Lim, C.T Ladoux, B |
Keywords: | myosin adenosine triphosphatase actin myosin adenosine triphosphatase Article cell proliferation controlled study epithelium cell force human human cell keratinocyte mechanics microscopy molecular model simulation tension tissue level traction force microscopy actin filament animal atomic force microscopy cell adhesion chemistry computer simulation confocal microscopy dog epithelium extracellular matrix MDCK cell line metabolism theoretical model tumor cell line Actin Cytoskeleton Actins Actomyosin Animals Cell Adhesion Cell Line, Tumor Cell Proliferation Computer Simulation Dogs Epithelium Extracellular Matrix Humans Madin Darby Canine Kidney Cells Microscopy, Atomic Force Microscopy, Confocal Models, Theoretical |
Issue Date: | 2015 | Publisher: | Nature Publishing Group | Citation: | Vedula, S.R.K, Peyret, G, Cheddadi, I, Chen, T, Brugués, A, Hirata, H, Lopez-Menendez, H, Toyama, Y, Neves De Almeida, L, Trepat, X, Lim, C.T, Ladoux, B (2015). Mechanics of epithelial closure over non-adherent environments. Nature Communications 6 : 6111. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms7111 | Abstract: | The closure of gaps within epithelia is crucial to maintain its integrity during biological processes such as wound healing and gastrulation. Depending on the distribution of extracellular matrix, gap closure occurs through assembly of multicellular actin-based contractile cables or protrusive activity of border cells into the gap. Here we show that the supracellular actomyosin contractility of cells near the gap edge exerts sufficient tension on the surrounding tissue to promote closure of non-adherent gaps. Using traction force microscopy, we observe that cell-generated forces on the substrate at the gap edge first point away from the centre of the gap and then increase in the radial direction pointing into the gap as closure proceeds. Combining with numerical simulations, we show that the increase in force relies less on localized purse-string contractility and more on large-scale remodelling of the suspended tissue around the gap. Our results provide a framework for understanding the assembly and the mechanics of cellular contractility at the tissue level. © 2015 Macmillan Publishers Limited. All rights reserved. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/175517 | ISSN: | 20411723 | DOI: | 10.1038/ncomms7111 |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1038_ncomms7111.pdf | 3.61 MB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.