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https://doi.org/10.1038/ncomms8683
Title: | Gap geometry dictates epithelial closure efficiency | Authors: | Ravasio, A Cheddadi, I Chen, T Pereira, T Ong, H.T Bertocchi, C Brugues, A Jacinto, A Kabla, A.J Toyama, Y Trepat, X Gov, N Neves De Almeida, L Ladoux, B |
Keywords: | fibronectin myosin adenosine triphosphatase myosin adenosine triphosphatase biological development cells and cell components computer simulation physiology tumor wounding actin filament actin polymerization animal cell Article cell adhesion cell interaction cell junction cell motility cell motion controlled study cross linking Drosophila melanogaster EC50 epithelium epithelium cell focal adhesion immunofluorescence in vitro study in vivo study lamellipodium laser surgery nonhuman randomized controlled trial wound closure animal computer simulation dog epithelium fluorescent antibody technique intravital microscopy low level laser therapy MDCK cell line metabolism microsurgery physiology wound healing Actomyosin Animals Cell Movement Computer Simulation Dogs Drosophila melanogaster Epithelial Cells Epithelium Fluorescent Antibody Technique In Vitro Techniques Intravital Microscopy Laser Therapy Madin Darby Canine Kidney Cells Microsurgery Wound Healing |
Issue Date: | 2015 | Publisher: | Nature Publishing Group | Citation: | Ravasio, A, Cheddadi, I, Chen, T, Pereira, T, Ong, H.T, Bertocchi, C, Brugues, A, Jacinto, A, Kabla, A.J, Toyama, Y, Trepat, X, Gov, N, Neves De Almeida, L, Ladoux, B (2015). Gap geometry dictates epithelial closure efficiency. Nature Communications 6 : 8683. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms8683 | Abstract: | Closure of wounds and gaps in tissues is fundamental for the correct development and physiology of multicellular organisms and, when misregulated, may lead to inflammation and tumorigenesis. To re-establish tissue integrity, epithelial cells exhibit coordinated motion into the void by active crawling on the substrate and by constricting a supracellular actomyosin cable. Coexistence of these two mechanisms strongly depends on the environment. However, the nature of their coupling remains elusive because of the complexity of the overall process. Here we demonstrate that epithelial gap geometry in both in vitro and in vivo regulates these collective mechanisms. In addition, the mechanical coupling between actomyosin cable contraction and cell crawling acts as a large-scale regulator to control the dynamics of gap closure. Finally, our computational modelling clarifies the respective roles of the two mechanisms during this process, providing a robust and universal mechanism to explain how epithelial tissues restore their integrity. © 2015 Macmillan Publishers Limited. All rights reserved. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/175499 | ISSN: | 20411723 | DOI: | 10.1038/ncomms8683 |
Appears in Collections: | Staff Publications Elements |
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