Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep15204
Title: A comparative genomics and reductive dehalogenase gene transcription study of two chloroethene-respiring bacteria, Dehalococcoides mccartyi strains MB and 11a
Authors: Low, A 
Shen, Z
Cheng, D 
Rogers, M.J 
Lee, P.K.H
He, J 
Keywords: bacterial protein
trichloroethylene
bacterial gene
bacterial genome
bacterium
comparative study
genetic transcription
genetics
genomics
halogenation
metabolism
procedures
upregulation
Bacteria
Bacterial Proteins
Genes, Bacterial
Genome, Bacterial
Genomics
Halogenation
Transcription, Genetic
Trichloroethylene
Up-Regulation
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Low, A, Shen, Z, Cheng, D, Rogers, M.J, Lee, P.K.H, He, J (2015). A comparative genomics and reductive dehalogenase gene transcription study of two chloroethene-respiring bacteria, Dehalococcoides mccartyi strains MB and 11a. Scientific Reports 5 : 15204. ScholarBank@NUS Repository. https://doi.org/10.1038/srep15204
Abstract: Genomes of two trichloroethene (TCE)-respiring Dehalococcoides (Dhc) mccartyi, strains MB and 11a, were sequenced to identify reductive dehalogenases (RDase) responsible for oraganohalide respiration. Transcription analyses were conducted to verify the roles of RDase subunit A genes (rdhA) in chloroethene respiration. Some interesting features of the strain MB draft genome include a large genome size, two CRISPR-cas type I systems, and 38 rdhA genes. Strain 11a has a stream-lined genome with 11 rdhA genes, of which nine are distinct. Quantitative real-time PCR transcription analysis of RDase gene transcripts showed that a single RDase gene, designated mbrA, was up-regulated upon exposure to TCE and no other RDase genes were considerably expressed in strain MB. A single RDase gene, designated vcrA, was up-regulated upon exposure to TCE and expressed at a steady level until all chloroethenes were completely dechlorinated to ethene at 147 h in strain 11a. Overall, this study reports the genomes of two distinct Dhc strains; both contain numerous uncharacterized RDase genes, but in each strain only one such gene was expressed highly during organohalide respiration.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175471
ISSN: 20452322
DOI: 10.1038/srep15204
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