Please use this identifier to cite or link to this item: https://doi.org/10.1111/jcmm.13589
Title: Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy
Authors: Yam, G.H.-F 
Teo, E.P.-W
Setiawan, M
Lovatt, M.J 
Yusoff, N.Z.B.M
Fuest, M
Goh, B.-T 
Mehta, J.S 
Keywords: 5' nucleotidase
activated leukocyte cell adhesion molecule
aldehyde dehydrogenase
aldehyde dehydrogenase isoenzyme 3 a1
angiopoietin 1
carbohydrate sulfotransferase 6
CD14 antigen
CD34 antigen
collagen type 8
cycline
endoglin
Hermes antigen
keratocan
lumican
n acetylglucosaminyltransferase
nestin
phalloidin
platelet endothelial cell adhesion molecule 1
receptor type tyrosine protein phosphatase C
sulfurtransferase
Thy 1 membrane glycoprotein
transcription factor FKHR
transcription factor GATA 4
transcription factor Sox10
transcription factor Sox2
unclassified drug
adolescent
adult
adult stem cell
Article
cell differentiation
cell migration
cell proliferation
cell regeneration
cellular distribution
confocal microscopy
controlled study
cornea opacity
cornea stroma cell
extracellular matrix
female
fibroblast
flow cytometry
gene expression
genetic association
human
human cell
human tissue
immunohistochemistry
male
neural crest cell
periodontal ligament
phenotype
postnatal growth
priority journal
real time polymerase chain reaction
regenerative medicine
RNA extraction
upregulation
adult stem cell
animal
cell lineage
cell motion
cornea
cornea cell
cornea disease
cytology
genetics
growth, development and aging
neural crest
pathology
periodontal ligament
pig
transplantation
Adult Stem Cells
Animals
Cell Differentiation
Cell Lineage
Cell Movement
Cornea
Corneal Diseases
Corneal Keratocytes
Humans
Neural Crest
Periodontal Ligament
Regenerative Medicine
Swine
Issue Date: 2018
Citation: Yam, G.H.-F, Teo, E.P.-W, Setiawan, M, Lovatt, M.J, Yusoff, N.Z.B.M, Fuest, M, Goh, B.-T, Mehta, J.S (2018). Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy. Journal of Cellular and Molecular Medicine 22 (6) : 3119-3132. ScholarBank@NUS Repository. https://doi.org/10.1111/jcmm.13589
Abstract: Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest. We harvested PDL cells from young adult teeth extracted because of non-functional or orthodontic reason and differentiated them towards CSK phenotype using a two-step protocol with spheroid formation followed by growth factor and cytokine induction in a stromal environment (human amnion stroma and porcine corneal stroma). Our results showed that the PDL-differentiated CSK-like cells expressed CSK markers (CD34, ALDH3A1, keratocan, lumican, CHST6, B3GNT7 and Col8A2) and had minimal expression of genes related to fibrosis and other lineages (vasculogenesis, adipogenesis, myogenesis, epitheliogenesis, neurogenesis and hematogenesis). Introduction of PDL spheroids into the stroma of porcine corneas resulted in extensive migration of cells inside the host stroma after 14-day organ culture. Their quiescent nature and uniform cell distribution resembled to that of mature CSKs inside the native stroma. Our results demonstrated the potential translation of PDL cells for regenerative corneal cell therapy for corneal opacities. © 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
Source Title: Journal of Cellular and Molecular Medicine
URI: https://scholarbank.nus.edu.sg/handle/10635/175381
ISSN: 1582-1838
DOI: 10.1111/jcmm.13589
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