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https://doi.org/10.1007/s00412-013-0423-z
Title: | Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes | Authors: | Gan, L Ladinsky, M.S Jensen, G.J |
Keywords: | article chromatin chromatin assembly and disassembly chromatin structure chromosome analysis chromosome rearrangement chromosome structure controlled study electron cryotomography electron tomography mitosis nonhuman nucleosome Ostreococcus Ostreococcus tauri polymer melt chromatin structure analysis Eukaryota Ostreococcus tauri Chlorophyta Chromatin Chromosomes Cryoelectron Microscopy Eukaryotic Cells Kinetochores Mitosis Nucleosomes |
Issue Date: | 2013 | Citation: | Gan, L, Ladinsky, M.S, Jensen, G.J (2013). Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes. Chromosoma 122 (5) : 377-386. ScholarBank@NUS Repository. https://doi.org/10.1007/s00412-013-0423-z | Abstract: | Chromatin organization is central to many conserved biological processes, but it is generally unknown how the underlying nucleosomes are arranged in situ. Here, we have used electron cryotomography to study chromatin in the picoplankton Ostreococcus tauri, the smallest known free-living eukaryote. By visualizing the nucleosome densities directly, we find that O. tauri chromosomes do not arrange into discrete, compact bodies or any other higher level of order. In contrast to the textbook 30-nm fiber model, O. tauri chromatin resembles a disordered assemblage of nucleosomes akin to the polymer melt model. This disorganized nucleosome arrangement has important implications for potentially conserved functions in tiny eukaryotes such as the clustering of nonhomologous chromosomes at the kinetochore during mitosis and the independent regulation of closely positioned adjacent genes. © 2013 The Author(s). | Source Title: | Chromosoma | URI: | https://scholarbank.nus.edu.sg/handle/10635/175328 | ISSN: | 0009-5915 | DOI: | 10.1007/s00412-013-0423-z |
Appears in Collections: | Staff Publications Elements |
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