Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00412-013-0423-z
Title: Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes
Authors: Gan, L 
Ladinsky, M.S
Jensen, G.J
Keywords: article
chromatin
chromatin assembly and disassembly
chromatin structure
chromosome analysis
chromosome rearrangement
chromosome structure
controlled study
electron cryotomography
electron tomography
mitosis
nonhuman
nucleosome
Ostreococcus
Ostreococcus tauri
polymer melt chromatin
structure analysis
Eukaryota
Ostreococcus tauri
Chlorophyta
Chromatin
Chromosomes
Cryoelectron Microscopy
Eukaryotic Cells
Kinetochores
Mitosis
Nucleosomes
Issue Date: 2013
Citation: Gan, L, Ladinsky, M.S, Jensen, G.J (2013). Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes. Chromosoma 122 (5) : 377-386. ScholarBank@NUS Repository. https://doi.org/10.1007/s00412-013-0423-z
Abstract: Chromatin organization is central to many conserved biological processes, but it is generally unknown how the underlying nucleosomes are arranged in situ. Here, we have used electron cryotomography to study chromatin in the picoplankton Ostreococcus tauri, the smallest known free-living eukaryote. By visualizing the nucleosome densities directly, we find that O. tauri chromosomes do not arrange into discrete, compact bodies or any other higher level of order. In contrast to the textbook 30-nm fiber model, O. tauri chromatin resembles a disordered assemblage of nucleosomes akin to the polymer melt model. This disorganized nucleosome arrangement has important implications for potentially conserved functions in tiny eukaryotes such as the clustering of nonhomologous chromosomes at the kinetochore during mitosis and the independent regulation of closely positioned adjacent genes. © 2013 The Author(s).
Source Title: Chromosoma
URI: https://scholarbank.nus.edu.sg/handle/10635/175328
ISSN: 0009-5915
DOI: 10.1007/s00412-013-0423-z
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