Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-017-17628-z
Title: Non-malignant epithelial cells preferentially proliferate from nasopharyngeal carcinoma biopsy cultured under conditionally reprogrammed conditions
Authors: Yu F. 
Lu Y.
Tao L. 
Jiang Y.-Y. 
Lin D.-C.
Wang L. 
Petersson F. 
Yoshiyama H.
Koeffler P.H.
Goh B.-C. 
Loh K.S. 
Keywords: 3T3 cell line
adult
aged
animal
biopsy
cell proliferation
coculture
dna mutational analysis
epithelium cell
evaluation study
feeder cell
female
genetics
human
male
middle aged
mouse
mutation
nasopharynx carcinoma
nasopharynx tumor
nuclear reprogramming
pathology
physiology
primary cell culture
procedures
tumor cell culture
3T3 Cells
Adult
Aged
Animals
Biopsy
Cell Proliferation
Cellular Reprogramming
Coculture Techniques
DNA Mutational Analysis
Epithelial Cells
Feeder Cells
Female
Humans
Male
Mice
Middle Aged
Mutation
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms
Primary Cell Culture
Tumor Cells, Cultured
Issue Date: 2017
Citation: Yu F., Lu Y., Tao L., Jiang Y.-Y., Lin D.-C., Wang L., Petersson F., Yoshiyama H., Koeffler P.H., Goh B.-C., Loh K.S. (2017). Non-malignant epithelial cells preferentially proliferate from nasopharyngeal carcinoma biopsy cultured under conditionally reprogrammed conditions. Scientific Reports 7 (1) : 17359. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-17628-z
Abstract: Nasopharyngeal carcinoma (NPC) is an invasive cancer with particularly high incidence in Southern China and Southeast Asia. The study of NPC is greatly hampered by the lack of reliable cell lines due to the loss of EBV genome and HeLa cell contamination. Conditional reprogramming (CR) cell culture technique has been reported for rapid and efficient establishment of patient derived normal and tumor cell cultures. The purpose of this study was to assess this method to culture NPC patient derived primary tumor cells. Using CR protocol, we demonstrated that epithelial cells could be efficiently cultured from normal (70%) and cancerous nasopharyngeal (46%) biopsies. However, by comparing with original tumors in terms of mutation and methylation profiles, epithelial cells derived from cancerous biopsy represented non malignant cells. Further, they exhibited stem like characteristics based on their cell surface proteins and could differentiate into pseudostratified epithelium in an air-liquid interface culture system. We conclude that CR method is a highly selective and useful method for growing non malignant nasopharyngeal epithelial cells. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175087
ISSN: 20452322
DOI: 10.1038/s41598-017-17628-z
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