Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/175075
Title: Lipid profiling of C. elegans strains administered pro-longevity drugs and drug combinations
Authors: Admasu, T.D
Batchu, K.C
Ng, L.F
Cazenave-Gassiot, A 
Wenk, M.R 
Gruber, J 
Keywords: Caenorhabditis elegans protein
DAF-7 protein, C elegans
Eat-2 protein, C elegans
nicotinic receptor
phosphatidylcholine
phosphatidylethanolamine
SBP-1 protein, C elegans
transcription factor
transforming growth factor beta
triacylglycerol
animal
Caenorhabditis elegans
caloric restriction
drug combination
drug effect
genetics
lipid metabolism
longevity
metabolism
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Caloric Restriction
Drug Combinations
Lipid Metabolism
Longevity
Phosphatidylcholines
Phosphatidylethanolamines
Receptors, Nicotinic
Transcription Factors
Transforming Growth Factor beta
Triglycerides
Issue Date: 2018
Citation: Admasu, T.D, Batchu, K.C, Ng, L.F, Cazenave-Gassiot, A, Wenk, M.R, Gruber, J (2018). Lipid profiling of C. elegans strains administered pro-longevity drugs and drug combinations. Scientific Data 5 : 180231. ScholarBank@NUS Repository.
Abstract: We report the effect of four lifespan modifying drugs and of synergistic combinations of these drugs on lipid profile in Caenorhabditis elegans. We employ ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to compare the abundance of lipid species in treated and control animals. Adult nematodes were treated with rapamycin, rifampicin, psora-4 and allantoin and combinations of these compounds and the resulting change in lipid profiles, specifically in those of triacylglycerol (TAG), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were determined. We quantified changes resulting from treatment with the drug combinations relative to untreated controls and relative to animals treated with each constituent single drugs. We further determined the dependence of changes in lipid profiles on genes known to affect lipid metabolism using strains carrying mutations in these pathways. In particular, we determined lipid profiles in a genetic model of caloric restriction (eat-2), a strain lacking homolog of TGF? (daf-7) and in a strain lacking the SREBP/sbp-1 transcription factor. © The Author(s) 2018.
Source Title: Scientific Data
URI: https://scholarbank.nus.edu.sg/handle/10635/175075
ISSN: 20524463
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