Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-018-26342-3
Title: Extraction and quantification of biofilm bacteria: Method optimized for urinary catheters
Authors: Mandakhalikar K.D. 
Rahmat J.N. 
Chiong E. 
Neoh K.G. 
Shen L. 
Tambyah P.A. 
Keywords: animal
biofilm
bladder catheterization
catheter infection
classification
Escherichia coli
Escherichia coli infection
female
growth, development and aging
human
indwelling catheter
isolation and purification
microbiology
mouse
pig
procedures
scanning electron microscopy
ultrastructure
urinary catheter
urinary tract infection
Animals
Biofilms
Catheter-Related Infections
Catheters, Indwelling
Escherichia coli
Escherichia coli Infections
Female
Humans
Mice
Microscopy, Electron, Scanning
Swine
Urinary Catheterization
Urinary Catheters
Urinary Tract Infections
Issue Date: 2018
Citation: Mandakhalikar K.D., Rahmat J.N., Chiong E., Neoh K.G., Shen L., Tambyah P.A. (2018). Extraction and quantification of biofilm bacteria: Method optimized for urinary catheters. Scientific Reports 8 (1) : 8069. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-26342-3
Abstract: Bacterial biofilms are responsible for the failure of many medical devices such as urinary catheters and are associated with many infectious and non-infectious complications. Preclinical and clinical evaluation of novel catheter coatings to prevent these infections needs to accurately quantify the bacterial load in the biofilm in vitro and ex vivo. There is currently no uniform gold standard for biofilm quantification for different surfaces and established biofilms. We have tried to establish a simple, accurate and reproducible method for extraction and measurement of biofilm bacteria on indwelling catheters, using a combination of vortexing and sonication. We demonstrate the usefulness of this method for catheters of different sizes - 3 Fr to 14 Fr - in vitro, in murine and porcine models, and indwelling in human clinical subjects. We also demonstrate consistent results with complex and polymicrobial biofilms. We believe that this standardized reproducible method will assist the assessment of biofilms in general and urological devices in particular in efforts to harness novel technologies to prevent healthcare associated infections. © 2018 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175021
ISSN: 20452322
DOI: 10.1038/s41598-018-26342-3
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