Please use this identifier to cite or link to this item: https://doi.org/10.3390/ijms151222028
Title: The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia
Authors: Fu G.H.
Liu F. 
Xia J.H.
Yue G.H. 
Keywords: actin related protein
actin related protein 5
adenosine triphosphate
amino acid
beta actin
complementary DNA
genomic DNA
messenger RNA
unclassified drug
vesicular inhibitory amino acid transporter
acute phase protein
carrier protein
fish protein
lipopolysaccharide-binding protein
membrane protein
Aeromonas hydrophila
animal experiment
animal tissue
Article
bacterial infection
bacterial strain
controlled study
disease resistance
exon
gene
gene expression
gene identification
gene mapping
gene sequence
gene structure
genetic analysis
genetic association
genotype
immune response
intron
lipopolysaccharide binding protein gene
nonhuman
nucleotide sequence
open reading frame
real time polymerase chain reaction
RNA extraction
single nucleotide polymorphism
Streptococcus agalactiae
Tilapia
upregulation
animal
chromosome map
computer simulation
disease resistance
DNA sequence
gene expression profiling
genetic linkage
genetics
genome
Gram negative infection
growth, development and aging
immunology
metabolism
microbiology
physiology
quantitative trait
Tilapia
Aeromonas hydrophila
Tilapia
Acute-Phase Proteins
Aeromonas hydrophila
Animals
Carrier Proteins
Chromosome Mapping
Computer Simulation
Disease Resistance
DNA, Complementary
Fish Proteins
Gene Expression Profiling
Genetic Linkage
Genome
Gram-Negative Bacterial Infections
Membrane Glycoproteins
Polymorphism, Single Nucleotide
Quantitative Trait, Heritable
Sequence Analysis, DNA
Tilapia
Issue Date: 2014
Citation: Fu G.H., Liu F., Xia J.H., Yue G.H. (2014). The LBP gene and its association with resistance to Aeromonas hydrophila in Tilapia. International Journal of Molecular Sciences 15 (12) : 22028-22041. ScholarBank@NUS Repository. https://doi.org/10.3390/ijms151222028
Abstract: Resistance to pathogens is important for the sustainability and profitability of food fish production. In immune-related genes, the lipopolysaccharide-binding protein (LBP) gene is an important mediator of the inflammatory reaction. We analyzed the cDNA and genomic structure of the LBP gene in tilapia. The full-length cDNA (1901 bp) of the gene contained a 1416 bp open reading frame, encoding 471 amino acid residues. Its genomic sequence was 5577 bp, comprising 15 exons and 14 introns. Under normal conditions, the gene was constitutively expressed in all examined tissues. The highest expression was detected in intestine and kidney. We examined the responses of the gene to challenges with two bacterial pathogens Streptcoccus agalactiae and Aeromonas hydrophila. The gene was significantly upregulated in kidney and spleen post-infection with S. agalactiae and A. hydrophila, respectively. However, the expression profiles of the gene after the challenge with the two pathogens were different. Furthermore, we identified three SNPs in the gene. There were significant associations (p < 0.05) of two of the three SNPs with the resistance to A. hydrophila, but not with the resistance to S. agalactiae or growth performance. These results suggest that the LBP gene is involved in the acute-phase immunologic response to the bacterial infections, and the responses to the two bacterial pathogens are different. The two SNPs associated with the resistance to A. hydrophila may be useful in the selection of tilapia resistant to A. hydrophila. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
Source Title: International Journal of Molecular Sciences
URI: https://scholarbank.nus.edu.sg/handle/10635/174647
ISSN: 1661-6596
DOI: 10.3390/ijms151222028
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