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https://doi.org/10.1038/s41467-017-00524-5
Title: | Topologically associating domains are ancient features that coincide with Metazoan clusters of extreme noncoding conservation | Authors: | Harmston, N Ing-Simmons, E Tan, G Perry, M Merkenschlager, M Lenhard, B |
Keywords: | conservation fly gene expression genetic analysis genome metazoan topology Drosophila human nonhuman regulator gene writing animal conserved sequence genetics genome genome size Metazoa spacer DNA Animals Conserved Sequence DNA, Intergenic Genes, Regulator Genome Genome Size Humans |
Issue Date: | 2017 | Publisher: | Nature Publishing Group | Citation: | Harmston, N, Ing-Simmons, E, Tan, G, Perry, M, Merkenschlager, M, Lenhard, B (2017). Topologically associating domains are ancient features that coincide with Metazoan clusters of extreme noncoding conservation. Nature Communications 8 (1) : 841. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-00524-5 | Abstract: | Developmental genes in metazoan genomes are surrounded by dense clusters of conserved noncoding elements (CNEs). CNEs exhibit unexplained extreme levels of sequence conservation, with many acting as developmental long-range enhancers. Clusters of CNEs define the span of regulatory inputs for many important developmental regulators and have been described previously as genomic regulatory blocks (GRBs). Their function and distribution around important regulatory genes raises the question of how they relate to 3D conformation of these loci. Here, we show that clusters of CNEs strongly coincide with topological organisation, predicting the boundaries of hundreds of topologically associating domains (TADs) in human and Drosophila. The set of TADs that are associated with high levels of noncoding conservation exhibit distinct properties compared to TADs devoid of extreme noncoding conservation. The close correspondence between extreme noncoding conservation and TADs suggests that these TADs are ancient, revealing a regulatory architecture conserved over hundreds of millions of years. © 2017 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174400 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-017-00524-5 |
Appears in Collections: | Elements Staff Publications |
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