Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-017-00524-5
Title: Topologically associating domains are ancient features that coincide with Metazoan clusters of extreme noncoding conservation
Authors: Harmston, N 
Ing-Simmons, E
Tan, G
Perry, M
Merkenschlager, M
Lenhard, B
Keywords: conservation
fly
gene expression
genetic analysis
genome
metazoan
topology
Drosophila
human
nonhuman
regulator gene
writing
animal
conserved sequence
genetics
genome
genome size
Metazoa
spacer DNA
Animals
Conserved Sequence
DNA, Intergenic
Genes, Regulator
Genome
Genome Size
Humans
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Harmston, N, Ing-Simmons, E, Tan, G, Perry, M, Merkenschlager, M, Lenhard, B (2017). Topologically associating domains are ancient features that coincide with Metazoan clusters of extreme noncoding conservation. Nature Communications 8 (1) : 841. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-00524-5
Abstract: Developmental genes in metazoan genomes are surrounded by dense clusters of conserved noncoding elements (CNEs). CNEs exhibit unexplained extreme levels of sequence conservation, with many acting as developmental long-range enhancers. Clusters of CNEs define the span of regulatory inputs for many important developmental regulators and have been described previously as genomic regulatory blocks (GRBs). Their function and distribution around important regulatory genes raises the question of how they relate to 3D conformation of these loci. Here, we show that clusters of CNEs strongly coincide with topological organisation, predicting the boundaries of hundreds of topologically associating domains (TADs) in human and Drosophila. The set of TADs that are associated with high levels of noncoding conservation exhibit distinct properties compared to TADs devoid of extreme noncoding conservation. The close correspondence between extreme noncoding conservation and TADs suggests that these TADs are ancient, revealing a regulatory architecture conserved over hundreds of millions of years. © 2017 The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/174400
ISSN: 2041-1723
DOI: 10.1038/s41467-017-00524-5
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